Fungal Microbiota in Air-conditioning Installed in Both Adult and Neonatal Intensive Treatment Units and Their Impact in Two University Hospitals of the Central Western Region, Mato Grosso, Brazil

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 2011 Mar 23

PMID 21424438

Citations 5

Authors

Sara de Almeida Alves Simoes

Diniz Pereira Leite Junior

Rosane Christine Hahn

Affiliations

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Abstract

To evaluate fungal microbiota in air-conditioning units installed in intensive care units in two university hospitals in Cuiaba city, Mato Grosso, central western region of Brazil, 525 solid environmental samples were collected, 285 from Hospital A and 240 from Hospital B. Collections were performed using sterile swabs on air-conditioning unit components: cooling coils, ventilators, and filters. Mycelial fungi identification was achieved by observation of the macroscopic and micromorphological characteristics in different culture mediums (maize meal, oatmeal and potato dextrose agars and malt extract) using the Ridell technique. Eleven genera and 27 distinct species belonging to the hyphomycetes and ascomycetes classes were isolated and identified. The most frequently detected genera in both hospitals were Aspergillus spp, Penicillium spp, and Cladosporium spp. Values for colony-forming units per gram were 64 and 75%, well above the limits recommended by Health Ministry resolution 176/00 at the locations selected for analysis in Hospitals A and B, respectively. In conclusion, evaluation of fungal microbiota in the air-conditioning units indirectly determined that the air quality was compromised in both university hospitals analyzed, which constitutes a risk factor for the acquisition of infection in the intensive care units.

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References

Anaissie E, Stratton S, Dignani M, Summerbell R, Rex J, Monson T . Pathogenic Aspergillus species recovered from a hospital water system: a 3-year prospective study. Clin Infect Dis. 2002; 34(6):780-9. DOI: 10.1086/338958. View

Rhame F . Prevention of nosocomial aspergillosis. J Hosp Infect. 1991; 18 Suppl A:466-72. DOI: 10.1016/0195-6701(91)90058-g. View

Hahn T, Cummings K, Michalek A, Lipman B, Segal B, McCarthy Jr P . Efficacy of high-efficiency particulate air filtration in preventing aspergillosis in immunocompromised patients with hematologic malignancies. Infect Control Hosp Epidemiol. 2002; 23(9):525-31. DOI: 10.1086/502101. View

Eickhoff T . Airborne nosocomial infection: a contemporary perspective. Infect Control Hosp Epidemiol. 1994; 15(10):663-72. DOI: 10.1086/646830. View

Oren I, Haddad N, Finkelstein R, Rowe J . Invasive pulmonary aspergillosis in neutropenic patients during hospital construction: before and after chemoprophylaxis and institution of HEPA filters. Am J Hematol. 2001; 66(4):257-62. DOI: 10.1002/ajh.1054. View

Singh N, Paterson D . Aspergillus infections in transplant recipients. Clin Microbiol Rev. 2005; 18(1):44-69. PMC: 544171. DOI: 10.1128/CMR.18.1.44-69.2005. View

Perdelli F, Sartini M, Spagnolo A, Dallera M, Lombardi R, Cristina M . A problem of hospital hygiene: the presence of aspergilli in hospital wards with different air-conditioning features. Am J Infect Control. 2006; 34(5):264-8. DOI: 10.1016/j.ajic.2005.12.004. View

Mobin M, do Amparo Salmito M . [Fungus microbiota in air conditioners in intensive care units in Teresina, Piauí]. Rev Soc Bras Med Trop. 2007; 39(6):556-9. DOI: 10.1590/s0037-86822006000600009. View

Doggett M . Characterization of fungal biofilms within a municipal water distribution system. Appl Environ Microbiol. 2000; 66(3):1249-51. PMC: 91974. DOI: 10.1128/AEM.66.3.1249-1251.2000. View

10.

Martins-Diniz J, da Silva R, Miranda E, Mendes-Giannini M . [Monitoring of airborne fungus and yeast species in a hospital unit]. Rev Saude Publica. 2005; 39(3):398-405. DOI: 10.1590/s0034-89102005000300010. View

11.

Hota B . Contamination, disinfection, and cross-colonization: are hospital surfaces reservoirs for nosocomial infection?. Clin Infect Dis. 2004; 39(8):1182-9. PMC: 7107941. DOI: 10.1086/424667. View

12.

Martins S, Moreira M, Furtado G, Marino C, Machado F, Wey S . Application of control measures for infections caused by multi-resistant gram-negative bacteria in intensive care unit patients. Mem Inst Oswaldo Cruz. 2004; 99(3):331-4. DOI: 10.1590/s0074-02762004000300017. View

13.

Hart C, Makin T . Legionella in hospitals: a review. J Hosp Infect. 1991; 18 Suppl A:481-9. DOI: 10.1016/0195-6701(91)90060-l. View

14.

Gioda A, de Aquino Neto F . [Comments on studies of industrial and non-industrial environments in Brazil: a comparative approach]. Cad Saude Publica. 2003; 19(5):1389-97. DOI: 10.1590/s0102-311x2003000500017. View

15.

Anaissie E, Stratton S, Dignani M, Lee C, Mahfouz T, Rex J . Cleaning patient shower facilities: a novel approach to reducing patient exposure to aerosolized Aspergillus species and other opportunistic molds. Clin Infect Dis. 2002; 35(8):E86-8. DOI: 10.1086/342305. View

16.

Fridkin S, Jarvis W . Epidemiology of nosocomial fungal infections. Clin Microbiol Rev. 1996; 9(4):499-511. PMC: 172907. DOI: 10.1128/CMR.9.4.499. View