Isolation of Methanobactin from the Spent Media of Methane-oxidizing Bacteria

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2011 Mar 23

PMID 21419927

Citations 24

Authors

Nathan L Bandow

Warren H Gallagher

Lee Behling

Dong W Choi

Jeremy D Semrau

Scott C Hartsel

Valerie S Gilles

Alan A DiSpirito

Affiliations

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Abstract

Chalkophores are low molecular mass modified peptides involved in copper acquisition in methane-oxidizing bacteria (MOB). A screening method for the detection of this copper-binding molecule is presented in Chapter 16. Here we describe methods to (1) maximize expression and secretion of chalkophores, (2) concentrate chalkophores from the spent media of MOB, and (3) purify chalkophores.

Citing Articles

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Crystal structure of MbnF: an NADPH-dependent flavin monooxygenase from Methylocystis strain SB2.

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Multiple Mechanisms for Copper Uptake by Methylosinus trichosporium OB3b in the Presence of Heterologous Methanobactin.

Peng P, Gu W, DiSpirito A, Semrau J mBio. 2022; 13(5):e0223922.

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MbnC Is Not Required for the Formation of the N-Terminal Oxazolone in the Methanobactin from Methylosinus trichosporium OB3b.

Dershwitz P, Gu W, Roche J, Kang-Yun C, Semrau J, Bobik T Appl Environ Microbiol. 2021; 88(2):e0184121.

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Two TonB-Dependent Transporters in Methylosinus trichosporium OB3b Are Responsible for Uptake of Different Forms of Methanobactin and Are Involved in the Canonical "Copper Switch".

Peng P, Kang-Yun C, Chang J, Gu W, DiSpirito A, Semrau J Appl Environ Microbiol. 2021; 88(1):e0179321.

PMID: 34669437 PMC: 8752135. DOI: 10.1128/AEM.01793-21.