Genetic Control of Amadori Product Degradation in Bacillus Subtilis Via Regulation of FrlBONMD Expression by FrlR

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2011 Mar 15

PMID 21398478

Citations 12

Authors

Veronika Maria Deppe

Stephanie Klatte

Johannes Bongaerts

Karl-Heinz Maurer

Timothy OConnell

Friedhelm Meinhardt

Affiliations

Soon will be listed here.

Abstract

Bacillus subtilis is capable of degrading fructosamines. The phosphorylation and the cleavage of the resulting fructosamine 6-phosphates is catalyzed by the frlD and frlB gene products, respectively. This study addresses the physiological importance of the frlBONMD genes (formerly yurPONML), revealing the necessity of their expression for growth on fructosamines and focusing on the complex regulation of the corresponding transcription unit. In addition to the known regulation by the global transcriptional regulator CodY, the frl genes are repressed by the convergently transcribed FrlR (formerly YurK). The latter causes repression during growth on substrates other than fructosamines. Additionally, we identified in the first intergenic region of the operon an FrlR binding site which is centrally located within a 38-bp perfect palindromic sequence. There is genetic evidence that this sequence, in combination with FrlR, contributes to the remarkable decrease in the transcription downstream of the first gene of the frl operon.

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