Imaging Interactions of Cationic Antimicrobial Peptides with Model Lipid Monolayers Using X-ray Spectromicroscopy

Overview

Journal Eur Biophys J

Specialty Biophysics

Date 2011 Mar 8

PMID 21380600

Citations 4

Authors

Bonnie O Leung

Adam P Hitchcock

Amy Won

Anatoli Ianoul

Andreas Scholl

Affiliations

Soon will be listed here.

Abstract

The interaction of antimicrobial peptide anoplin with 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] lipid monolayers was imaged with atomic force microscopy, scanning transmission X-ray microscopy, and X-ray photoemission electron microscopy. X-ray absorption spectromicroscopy of the surface revealed the domains of the phase-segregated surface to be composed of 98(±5)% lipid while the matrix consisted of a ~50:50 lipid-peptide mixture. We show X-ray spectromicroscopy to be a valuable quantitative tool for label-free imaging of lipid monolayers with antimicrobial peptides at a lateral spatial resolution below 80 nm.

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