Nuclear Proteins Bind Conserved Elements in the Abscisic Acid-responsive Promoter of a Rice Rab Gene

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Feb 1

PMID 2137613

Citations 150

Authors

J Mundy

K Yamaguchi-Shinozaki

N H Chua

Affiliations

Soon will be listed here.

Abstract

We have previously shown that the expression of a rice gene, rab-16A, is responsive to abscisic acid (ABA) and osmotic stress in plant tissues and cultured suspension cells. We demonstrate here that transcriptional elements between -294 and -52 of this gene are sufficient to confer ABA-dependent expression on the chloramphenicol acetyltransferase reporter gene in rice protoplasts. Sequence motifs within this 242-base-pair region of the rab-16A gene are conserved among the 5' upstream regions of other ABA-responsive genes. Gel retardation and DNAse I experiments show nuclear factor(s) binding to these sequences. This correlative data indicate that these motifs are involved in the transcription of the rab genes and suggest that they may be ABA-responsive-elements (ABREs).

Citing Articles

Genome-Wide Identification and Analysis of Transcription Factor Family Genes Involved in Cold Stress Tolerance in Winter Rapeseed ( L.).

Xu Y, Ma L, Zeng X, Xu Y, Tao X, Fahim A Int J Mol Sci. 2025; 25(24.

PMID: 39769355 PMC: 11678751. DOI: 10.3390/ijms252413592.

Genome-wide characterization of TCP family and their potential roles in abiotic stress resistance of oat ( L.).

Pan J, Ju Z, Ma X, Duan L, Jia Z Front Plant Sci. 2024; 15:1382790.

PMID: 38654900 PMC: 11036127. DOI: 10.3389/fpls.2024.1382790.

Genomic and Expression Analysis of Cassava ( Crantz) Chalcone Synthase Genes in Defense against Infestation.

Yang Y, Liu M, Huang Z Genes (Basel). 2024; 15(3).

PMID: 38540395 PMC: 10970205. DOI: 10.3390/genes15030336.

Regulatory networks in plant responses to drought and cold stress.

Kim J, Kidokoro S, Yamaguchi-Shinozaki K, Shinozaki K Plant Physiol. 2024; 195(1):170-189.

PMID: 38514098 PMC: 11060690. DOI: 10.1093/plphys/kiae105.

Analysis of the Tomato Gene Family and Study of the Stress Resistance Function of .

Su A, Ge S, Zhou B, Wang Z, Zhou L, Zhang Z Plants (Basel). 2023; 12(15).

PMID: 37571015 PMC: 10421145. DOI: 10.3390/plants12152862.

References

Singh N, Larosa P, Handa A, Hasegawa P, Bressan R . Hormonal regulation of protein synthesis associated with salt tolerance in plant cells. Proc Natl Acad Sci U S A. 1987; 84(3):739-43. PMC: 304291. DOI: 10.1073/pnas.84.3.739. View

Ettlinger C, Lehle L . Auxin induces rapid changes in phosphatidylinositol metabolites. Nature. 1988; 331(6152):176-8. DOI: 10.1038/331176a0. View

Briggs M, Kadonaga J, Bell S, Tjian R . Purification and biochemical characterization of the promoter-specific transcription factor, Sp1. Science. 1986; 234(4772):47-52. DOI: 10.1126/science.3529394. View

Johnson P, McKnight S . Eukaryotic transcriptional regulatory proteins. Annu Rev Biochem. 1989; 58:799-839. DOI: 10.1146/annurev.bi.58.070189.004055. View

Hicks G, Rayle D, Jones A, Lomax T . Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin. Proc Natl Acad Sci U S A. 1989; 86:4948-52. PMC: 297533. DOI: 10.1073/pnas.86.13.4948. View

Beato M . Gene regulation by steroid hormones. Cell. 1989; 56(3):335-44. DOI: 10.1016/0092-8674(89)90237-7. View

MORTENSON E, Dreyfuss G . RNP in maize protein. Nature. 1989; 337(6205):312. DOI: 10.1038/337312b0. View

Schroeder J, Hedrich R . Involvement of ion channels and active transport in osmoregulation and signaling of higher plant cells. Trends Biochem Sci. 1989; 14(5):187-92. DOI: 10.1016/0968-0004(89)90272-7. View

Koornneef M, Hanhart C, Hilhorst H, Karssen C . In Vivo Inhibition of Seed Development and Reserve Protein Accumulation in Recombinants of Abscisic Acid Biosynthesis and Responsiveness Mutants in Arabidopsis thaliana. Plant Physiol. 1989; 90(2):463-9. PMC: 1061747. DOI: 10.1104/pp.90.2.463. View

10.

Goday A, Sanchez-Martinez D, Gomez J, Puigdomenech P, Pages M . Gene Expression in Developing Zea mays Embryos: Regulation by Abscisic Acid of a Highly Phosphorylated 23- to 25-kD Group of Proteins. Plant Physiol. 1988; 88(3):564-9. PMC: 1055625. DOI: 10.1104/pp.88.3.564. View

11.

Gomez J, Sanchez-Martinez D, Stiefel V, Rigau J, Puigdomenech P, Pages M . A gene induced by the plant hormone abscisic acid in response to water stress encodes a glycine-rich protein. Nature. 1988; 334(6179):262-4. DOI: 10.1038/334262a0. View

12.

Dente L, Cesareni G, Cortese R . pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 1983; 11(6):1645-55. PMC: 325826. DOI: 10.1093/nar/11.6.1645. View

13.

Mohapatra S, Poole R, Dhindsa R . Abscisic Acid-regulated gene expression in relation to freezing tolerance in alfalfa. Plant Physiol. 1988; 87(2):468-73. PMC: 1054776. DOI: 10.1104/pp.87.2.468. View

14.

Finkelstein R, Tenbarge K, Shumway J, Crouch M . Role of ABA in Maturation of Rapeseed Embryos. Plant Physiol. 1985; 78(3):630-6. PMC: 1064789. DOI: 10.1104/pp.78.3.630. View

15.

Deutsch P, Hoeffler J, Jameson J, Habener J . Cyclic AMP and phorbol ester-stimulated transcription mediated by similar DNA elements that bind distinct proteins. Proc Natl Acad Sci U S A. 1988; 85(21):7922-6. PMC: 282325. DOI: 10.1073/pnas.85.21.7922. View

16.

Mundy J, Chua N . Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J. 1988; 7(8):2279-86. PMC: 457090. DOI: 10.1002/j.1460-2075.1988.tb03070.x. View

17.

Lam E, Benfey P, Gilmartin P, Fang R, Chua N . Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci U S A. 1989; 86(20):7890-4. PMC: 298177. DOI: 10.1073/pnas.86.20.7890. View

18.

Mundy J, Hejgaard J, Hansen A, Hallgren L, Jorgensen K, Munck L . Differential synthesis in vitro of barley aleurone and starchy endosperm proteins. Plant Physiol. 1986; 81(2):630-6. PMC: 1075389. DOI: 10.1104/pp.81.2.630. View