Dynamic Clamp with StdpC Software

Overview

Journal Nat Protoc

Specialties Biology
Pathology
Science

Date 2011 Mar 5

PMID 21372819

Citations 19

Authors

Ildiko Kemenes

Vincenzo Marra

Michael Crossley

David Samu

Kevin Staras

Gyorgy Kemenes

Thomas Nowotny

Affiliations

Soon will be listed here.

Abstract

Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, for spike timing-dependent plasticity clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real-time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments through an intuitive and powerful interface with a minimal initial lead time of a few hours. After initial configuration, experimental results can be generated within minutes of establishing cell recording.

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