Production of HIV Particles is Regulated by Altering Sub-cellular Localization and Dynamics of Rev Induced by Double-strand RNA Binding Protein

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Mar 3

PMID 21364984

Citations 6

Authors

Silvio Urcuqui-Inchima

Claudia Patino

Ximena Zapata

Maria Patricia Garcia

Jose Arteaga

Christophe Chamot

Ajit Kumar

Daniele Hernandez-Verdun

Affiliations

Soon will be listed here.

Abstract

Human immunodeficiency virus (HIV)-1 encoded Rev is essential for export from the nucleus to the cytoplasm, of unspliced and singly spliced transcripts coding for structural and nonstructural viral proteins. This process is spatially and temporally coordinated resulting from the interactions between cellular and viral proteins. Here we examined the effects of the sub-cellular localization and dynamics of Rev on the efficiency of nucleocytoplasmic transport of HIV-1 Gag transcripts and virus particle production. Using confocal microscopy and fluorescence recovery after bleaching (FRAP), we report that NF90ctv, a cellular protein involved in Rev function, alters both the sub-cellular localization and dynamics of Rev in vivo, which drastically affects the accumulation of the viral protein p24. The CRM1-dependent nuclear export of Gag mRNA linked to the Rev Response Element (RRE) is dependent on specific domains of the NF90ctv protein. Taken together, our results demonstrate that the appropriate intracellular localization and dynamics of Rev could regulate Gag assembly and HIV-1 replication.

Citing Articles

Cytoplasmic Amplification of Transcriptional Noise Generates Substantial Cell-to-Cell Variability.

Hansen M, Desai R, Simpson M, Weinberger L Cell Syst. 2018; 7(4):384-397.e6.

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CRM1 Inhibitors for Antiviral Therapy.

Mathew C, Ghildyal R Front Microbiol. 2017; 8:1171.

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Novel interactions between the HTLV antisense proteins HBZ and APH-2 and the NFAR protein family: Implications for the HTLV lifecycles.

Murphy J, Hall W, Ratner L, Sheehy N Virology. 2016; 494:129-42.

PMID: 27110706 PMC: 4924524. DOI: 10.1016/j.virol.2016.04.012.

NF45 and NF90 Bind HIV-1 RNA and Modulate HIV Gene Expression.

Li Y, Belshan M Viruses. 2016; 8(2).

PMID: 26891316 PMC: 4776202. DOI: 10.3390/v8020047.

Proteomic analysis of early HIV-1 nucleoprotein complexes.

Schweitzer C, Jagadish T, Haverland N, Ciborowski P, Belshan M J Proteome Res. 2013; 12(2):559-72.

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