Impact of the DNA Methyltransferases Expression on the Methylation Status of Apoptosis-associated Genes in Glioblastoma Multiforme
Overview
General Medicine
Authors
Affiliations
Disruption of apoptosis is considered as an important factor aiding tumorigenesis, and aberrant DNA methylation of apoptosis-associated genes could be an important and significant mechanism through which tumor cells avoid apoptosis. However, little is known about (1) the impact of methylation status of apoptosis-associated genes on the presence of apoptosis evasion phenotype in glioma; and (2) the molecular mechanism governing the aberrant methylation of apoptosis-associated genes in glioma. By analyzing human glioma biopsies, we first show that low level of apoptosis in tumor is correlated with aberrant methylation of the bcl-2, bax and XAF-1 genes, but not with the aberrant methylation of the bcl-w, survivin, TMS1, caspase-8 and HRK genes. Our work also indicates that the expression levels of DNA methyltransferase 1 (Dnmt1), Dnmt3b and Dnmt1/Dnmt3a coregulate the methylation status of survivin, TMS1 and caspase-8, whereas no correlation was observed between the expression level of Dnmts and the methylation status of the bcl-w, bcl-2, bax, XAF-1 and HRK genes. Thus, these results indicate that the epigenetic regulation of some apoptosis-regulated genes could dictate whether glioma harbors the apoptosis evasion phenotype, and provide some bases to the identification of the methylation machineries of apoptosis-associated genes for which the Dnmt expression acts as a limiting factor.
Zahoor A, Khazer R, Mehraj I, Gani U, Fayaz F, Khanday F Funct Integr Genomics. 2025; 25(1):50.
PMID: 40024973 DOI: 10.1007/s10142-025-01552-x.
Gene Expression Regulation and the Signal Transduction of Programmed Cell Death.
Saqirile , Deng Y, Li K, Yan W, Li K, Wang C Curr Issues Mol Biol. 2024; 46(9):10264-10298.
PMID: 39329964 PMC: 11430711. DOI: 10.3390/cimb46090612.
The role of saponins on anti-cancer: The potential mechanism and molecular targets.
Ke F, Zhang R, Chen R, Guo X, Song C, Gao X Heliyon. 2024; 10(17):e37323.
PMID: 39296108 PMC: 11407946. DOI: 10.1016/j.heliyon.2024.e37323.
Bora Yildiz C, Kundu T, Gehrmann J, Koesling J, Ravaei A, Wolff P Epigenetics Chromatin. 2023; 16(1):42.
PMID: 37880732 PMC: 10601256. DOI: 10.1186/s13072-023-00516-4.
UXT at the crossroads of cell death, immunity and neurodegenerative diseases.
Han P, Mo S, Wang Z, Xu J, Fu X, Tian Y Front Oncol. 2023; 13:1179947.
PMID: 37152054 PMC: 10154696. DOI: 10.3389/fonc.2023.1179947.