Transforming Growth Factor-beta Increases the Expression of Vascular Smooth Muscle Cell Markers in Human Multi-lineage Progenitor Cells

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2011 Mar 2

PMID 21358594

Citations 6

Authors

Hui Yang

Lidong Zhang

Sarah M Weakley

Peter H Lin

Qizhi Yao

Changyi Chen

Affiliations

Soon will be listed here.

Abstract

Background: Vascular smooth muscle cell (SMC) differentiation is an essential component of vascular repair and tissue engineering. However, currently used cell models for the study of SMC differentiation have several limitations. Multi-lineage progenitor cells (MLPCs) originate from human umbilical cord blood and are cloned from a single cell. The object of this study was to investigate whether MLPCs could differentiate into SMCs in vitro with induction by transforming growth factor beta1 (TGF-beta1).

Material/methods: MLPCs were treated without or with TGF-beta1 (1 and 5 ng/mL) in mesenchymal stem cell media plus 1% FBS for 7 days. Total RNA was isolated from the MLPCs, and semi-quantitative real-time PCR was performed to test the following mRNA levels: early and late phase SMC-specific markers, two endothelial cell (EC)-specific markers, endothelial progenitor cell (EPC) marker CD34, TGF-beta1 accessory protein CD105, and adhesion molecule CD146.

Results: TGF-beta1 (1 ng/mL) significantly increased the mRNA levels of SMC-specific markers SM22α, calponin-1, SM α-actin, caldesmon, tropomyosin and MLCK as well as adhesion molecule CD146. The mRNA levels of EC-specific markers VE-cadherin and VEGFR-2, EPC marker CD34 and TGF-beta1 accessory protein CD105 were decreased significantly, after MLPC were treated with TGF-beta1 (1 ng/mL). TGF-beta1 at 5 ng/mL showed similar effect on the expression of these genes.

Conclusions: This study demonstrates that in the presence of TGF-beta1, MLPCs undergo SMC lineage differentiation indicating that MLPCs are a promising cell model for SMC lineage differentiation studies, which may contribute to advances in vascular repair and tissue engineering.

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References

Yamamoto K, Takahashi T, Asahara T, Ohura N, Sokabe T, Kamiya A . Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress. J Appl Physiol (1985). 2003; 95(5):2081-8. DOI: 10.1152/japplphysiol.00232.2003. View

Becker C, Laeufer T, Arikkat J, Jakse G . TGFbeta-1 and epithelial-mesenchymal interactions promote smooth muscle gene expression in bone marrow stromal cells: possible application in therapies for urological defects. Int J Artif Organs. 2008; 31(11):951-9. DOI: 10.1177/039139880803101105. View

Anfosso F, Bardin N, Frances V, Vivier E, Camoin-Jau L, Sampol J . Activation of human endothelial cells via S-endo-1 antigen (CD146) stimulates the tyrosine phosphorylation of focal adhesion kinase p125(FAK). J Biol Chem. 1998; 273(41):26852-6. DOI: 10.1074/jbc.273.41.26852. View

Sinha S, Hoofnagle M, Kingston P, McCanna M, Owens G . Transforming growth factor-beta1 signaling contributes to development of smooth muscle cells from embryonic stem cells. Am J Physiol Cell Physiol. 2004; 287(6):C1560-8. DOI: 10.1152/ajpcell.00221.2004. View

Simper D, Stalboerger P, Panetta C, Wang S, Caplice N . Smooth muscle progenitor cells in human blood. Circulation. 2002; 106(10):1199-204. DOI: 10.1161/01.cir.0000031525.61826.a8. View

Caglayan E, Romeo G, Kappert K, Odenthal M, Sudkamp M, Body S . Profilin-1 is expressed in human atherosclerotic plaques and induces atherogenic effects on vascular smooth muscle cells. PLoS One. 2010; 5(10):e13608. PMC: 2963617. DOI: 10.1371/journal.pone.0013608. View

Wrana J, Attisano L, Wieser R, Ventura F, Massague J . Mechanism of activation of the TGF-beta receptor. Nature. 1994; 370(6488):341-7. DOI: 10.1038/370341a0. View

Carmeliet P, Collen D . Transgenic mouse models in angiogenesis and cardiovascular disease. J Pathol. 2000; 190(3):387-405. DOI: 10.1002/(SICI)1096-9896(200002)190:3<387::AID-PATH595>3.0.CO;2-R. View

Hirschi K, Rohovsky S, DAmore P . PDGF, TGF-beta, and heterotypic cell-cell interactions mediate endothelial cell-induced recruitment of 10T1/2 cells and their differentiation to a smooth muscle fate. J Cell Biol. 1998; 141(3):805-14. PMC: 2132737. DOI: 10.1083/jcb.141.3.805. View

10.

Meyrick B . The pathology of pulmonary artery hypertension. Clin Chest Med. 2001; 22(3):393-404, vii. DOI: 10.1016/s0272-5231(05)70279-3. View

11.

Hristov M, Erl W, Weber P . Endothelial progenitor cells: mobilization, differentiation, and homing. Arterioscler Thromb Vasc Biol. 2003; 23(7):1185-9. DOI: 10.1161/01.ATV.0000073832.49290.B5. View

12.

Campard D, Lysy P, Najimi M, Sokal E . Native umbilical cord matrix stem cells express hepatic markers and differentiate into hepatocyte-like cells. Gastroenterology. 2008; 134(3):833-48. DOI: 10.1053/j.gastro.2007.12.024. View

13.

Chen S, Lechleider R . Transforming growth factor-beta-induced differentiation of smooth muscle from a neural crest stem cell line. Circ Res. 2004; 94(9):1195-202. DOI: 10.1161/01.RES.0000126897.41658.81. View

14.

Espana E, Kawakita T, Liu C, Tseng S . CD-34 expression by cultured human keratocytes is downregulated during myofibroblast differentiation induced by TGF-beta1. Invest Ophthalmol Vis Sci. 2004; 45(9):2985-91. DOI: 10.1167/iovs.04-0201. View

15.

Ouhtit A, Gaur R, Abd Elmageed Z, Fernando A, Thouta R, Trappey A . Towards understanding the mode of action of the multifaceted cell adhesion receptor CD146. Biochim Biophys Acta. 2009; 1795(2):130-6. DOI: 10.1016/j.bbcan.2009.01.002. View

16.

Lien S, Usami S, Chien S, Chiu J . Phosphatidylinositol 3-kinase/Akt pathway is involved in transforming growth factor-beta1-induced phenotypic modulation of 10T1/2 cells to smooth muscle cells. Cell Signal. 2005; 18(8):1270-8. DOI: 10.1016/j.cellsig.2005.10.013. View

17.

Derynck R, Feng X . TGF-beta receptor signaling. Biochim Biophys Acta. 1997; 1333(2):F105-50. DOI: 10.1016/s0304-419x(97)00017-6. View

18.

Watabe T, Yamashita J, Mishima K, Miyazono K . TGF-beta signaling in embryonic stem cell-derived endothelial cells. Methods Mol Biol. 2006; 330:341-51. DOI: 10.1385/1-59745-036-7:341. View

19.

Chen S, Crawford M, Day R, Briones V, Leader J, Jose P . RhoA modulates Smad signaling during transforming growth factor-beta-induced smooth muscle differentiation. J Biol Chem. 2005; 281(3):1765-70. PMC: 1831550. DOI: 10.1074/jbc.M507771200. View

20.

Hamilton D, Maul T, Vorp D . Characterization of the response of bone marrow-derived progenitor cells to cyclic strain: implications for vascular tissue-engineering applications. Tissue Eng. 2004; 10(3-4):361-9. DOI: 10.1089/107632704323061726. View