Culturing Human Embryonic Stem Cells in Feeder-free Conditions

Overview

Journal CSH Protoc

Specialty Biomedical Engineering

Date 2011 Mar 2

PMID 21356913

Citations 5

Authors

Sohyun L McElroy

Renee A Reijo Pera

Affiliations

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Abstract

INTRODUCTIONHuman embryonic stem cells (hESCs) have the potential to differentiate into all three germ layers and proliferate in long-term culture in vitro. hESCs can provide a cell source for the testing of novel therapies, drug screening, and functional genomics applications. Undifferentiated hESCs can be maintained and proliferated on mouse embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs without feeder cell contamination.

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