Exquisite Binding Specificity of Sclerotium Rolfsii Lectin Toward TF-related O-linked Mucin-type Glycans

Overview

Journal Glycoconj J

Publisher Springer

Specialties Biochemistry
Endocrinology

Date 2011 Feb 25

PMID 21347715

Citations 6

Authors

Vishwanath B Chachadi

Shashikala R Inamdar

Lu-Gang Yu

Jonathan M Rhodes

Bale M Swamy

Affiliations

Soon will be listed here.

Abstract

Sclerotium rolfsii lectin (SRL), a secretory protein from the soil borne phytopathogenic fungus Sclerotium rolfsii, has shown in our previous studies to bind strongly to the oncofetal Thomson-Friedenreich carbohydrate (Galβ1-3GalNAc-ser/thr, T or TF) antigen. TF antigen is widely expressed in many types of human cancers and the strong binding of SRL toward such a cancer-associated carbohydrate structure led us to characterize the carbohydrate binding specificity of SRL. Glycan array analysis, which included 285 glycans, shows exclusive binding of SRL to the O-linked mucin type but not N-linked glycans and amongst the mucin type O-glycans, lectin recognizes only mucin core 1, core 2 and weakly core 8 but not to other mucin core structures. It binds with high specificity to "α-anomers" but not the "β-anomers" of the TF structure. The axial C4-OH group of GalNAc and C2-OH group of Gal is both essential for SRL interaction with TF disaccharide, and substitution on C3 of galactose by sulfate or sialic acid or N-acetylglucosamine, significantly enhances the avidity of the lectin. SRL differs in its binding to TF structures compared to other known TF-binding lectins such as the Arachis hypogea (peanut) agglutinin, Agaricus bisporus (mushroom) lectin, Jackfruit, Artocarpus integrifolia (jacalin) and Amaranthus caudatus (Amaranthin) lectin. Thus, SRL has unique carbohydrate-binding specificity toward TF-related O-linked carbohydrate structures. Such a binding specificity will make this lectin a very useful tool in future structural as well as functional analysis of the cellular glycans in cancer studies.

Citing Articles

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Gurav M, Manasa J, Sanji A, Megalamani P, Chachadi V Glycoconj J. 2024; 41(4-5):301-322.

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Sclerotium rolfsii lectin induces opposite effects on normal PBMCs and leukemic Molt-4 cells by recognising TF antigen and its variants as receptors.

Chachadi V, Pujari R, Shastry P, Swamy B, Inamdar S Glycoconj J. 2020; 37(2):251-261.

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Molecular Cloning, Carbohydrate Specificity and the Crystal Structure of Two Sclerotium rolfsii Lectin Variants.

Peppa V, Venkat H, Kantsadi A, Inamdar S, Bhat G, Eligar S Molecules. 2015; 20(6):10848-65.

PMID: 26076107 PMC: 6272482. DOI: 10.3390/molecules200610848.

Sclerotium rolfsii lectin induces stronger inhibition of proliferation in human breast cancer cells than normal human mammary epithelial cells by induction of cell apoptosis.

Savanur M, Eligar S, Pujari R, Chen C, Mahajan P, Borges A PLoS One. 2014; 9(11):e110107.

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Sclerotium rolfsii lectin inhibits proliferation and induces apoptosis in human ovarian cancer cell line PA-1.

Eligar S, Pujari R, Swamy B, Shastry P, Inamdar S Cell Prolif. 2012; 45(5):397-403.

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