Microscale Plasma-initiated Patterning of Electrospun Polymer Scaffolds

Overview

Journal Colloids Surf B Biointerfaces

Publisher Elsevier

Specialty Chemistry

Date 2011 Feb 25

PMID 21345656

Authors

Roberto Delgado-Rivera

Jeremy Griffin

Christopher L Ricupero

Martin Grumet

Sally Meiners

Kathryn E Uhrich

Affiliations

Soon will be listed here.

Abstract

Microscale plasma-initiated patterning (μPIP) is a novel micropatterning technique used to create biomolecular micropatterns on polymer surfaces. The patterning method uses a polydimethylsiloxane (PDMS) stamp to selectively protect regions of an underlying substrate from oxygen plasma treatment resulting in hydrophobic and hydrophilic regions. Preferential adsorption of the biomolecules onto either the plasma-exposed (hydrophilic) or plasma-protected (hydrophobic) regions leads to the biomolecular micropatterns. In the current work, laminin-1 was applied to an electrospun polyamide nanofibrillar matrix following plasma treatment. Radial glial clones (neural precursors) selectively adhered to these patterned matrices following the contours of proteins on the surface. This work demonstrates that textured surfaces, such as nanofibrillar scaffolds, can be micropatterned to provide external chemical cues for cellular organization.

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