Antibacterial Compounds from Marine Vibrionaceae Isolated on a Global Expedition

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2011 Feb 23

PMID 21339958

Citations 45

Authors

Matthias Wietz

Maria Mansson

Charlotte H Gotfredsen

Thomas O Larsen

Lone Gram

Affiliations

Soon will be listed here.

Abstract

On a global research expedition, over 500 bacterial strains inhibitory towards pathogenic bacteria were isolated. Three hundred of the antibacterial strains were assigned to the Vibrionaceae family. The purpose of the present study was to investigate the phylogeny and bioactivity of five Vibrionaceae strains with pronounced antibacterial activity. These were identified as Vibrio coralliilyticus (two strains), V. neptunius (two strains), and Photobacterium halotolerans (one strain) on the basis of housekeeping gene sequences. The two related V. coralliilyticus and V. neptunius strains were isolated from distant oceanic regions. Chemotyping by LC-UV/MS underlined genetic relationships by showing highly similar metabolite profiles for each of the two V. coralliilyticus and V. neptunius strains, respectively, but a unique profile for P. halotolerans. Bioassay-guided fractionation identified two known antibiotics as being responsible for the antibacterial activity; andrimid (from V. coralliilyticus) and holomycin (from P. halotolerans). Despite the isolation of already known antibiotics, our findings show that marine Vibrionaceae are a resource of antibacterial compounds and may have potential for future natural product discovery.

Citing Articles

Oceanic Breakthroughs: Marine-Derived Innovations in Vaccination, Therapy, and Immune Health.

Gamberi C, Leverette C, Davis A, Ismail M, Piccialli I, Borbone N Vaccines (Basel). 2024; 12(11).

PMID: 39591167 PMC: 11598900. DOI: 10.3390/vaccines12111263.

Link between bacterial communities and contrasted loads in ectoparasitic monogeneans from the external mucus of two wild sparid species (Teleostei).

Revault J, Desdevises Y, Magnanou E Anim Microbiome. 2024; 6(1):42.

PMID: 39080784 PMC: 11290237. DOI: 10.1186/s42523-024-00329-0.

Comparative Genomic Analyses of the Genus Illuminate Biosynthetic Gene Clusters Associated with Antagonism.

Lau N, Heng W, Miswan N, Azami N, Furusawa G Int J Mol Sci. 2022; 23(17).

PMID: 36077108 PMC: 9456166. DOI: 10.3390/ijms23179712.

Solonamides, a Group of Cyclodepsipeptides, Influence Motility in the Native Producer Photobacterium galatheae S2753.

Zhang S, Lindqvist L, Isbrandt T, Borre I, Wibowo M, Nielsen M Appl Environ Microbiol. 2022; 88(17):e0110522.

PMID: 36000852 PMC: 9469707. DOI: 10.1128/aem.01105-22.

Predatory bacteria in the haemolymph of the cultured spiny lobster .

Ooi M, Goulden E, Smith G, Bridle A Microbiology (Reading). 2021; 167(11).

PMID: 34846286 PMC: 8743626. DOI: 10.1099/mic.0.001113.

References

Taylor M, Radax R, Steger D, Wagner M . Sponge-associated microorganisms: evolution, ecology, and biotechnological potential. Microbiol Mol Biol Rev. 2007; 71(2):295-347. PMC: 1899876. DOI: 10.1128/MMBR.00040-06. View

Vynne N, Mansson M, Nielsen K, Gram L . Bioactivity, chemical profiling, and 16S rRNA-based phylogeny of Pseudoalteromonas strains collected on a global research cruise. Mar Biotechnol (NY). 2011; 13(6):1062-73. DOI: 10.1007/s10126-011-9369-4. View

Jin M, Fischbach M, Clardy J . A biosynthetic gene cluster for the acetyl-CoA carboxylase inhibitor andrimid. J Am Chem Soc. 2006; 128(33):10660-1. PMC: 2529255. DOI: 10.1021/ja063194c. View

Heindl H, Wiese J, Thiel V, Imhoff J . Phylogenetic diversity and antimicrobial activities of bryozoan-associated bacteria isolated from Mediterranean and Baltic Sea habitats. Syst Appl Microbiol. 2010; 33(2):94-104. DOI: 10.1016/j.syapm.2009.12.002. View

Chien C, Chen C, Choi M, Kung S, Wei Y . Production of poly-beta-hydroxybutyrate (PHB) by Vibrio spp. isolated from marine environment. J Biotechnol. 2007; 132(3):259-63. DOI: 10.1016/j.jbiotec.2007.03.002. View

Kind T, Fiehn O . Metabolomic database annotations via query of elemental compositions: mass accuracy is insufficient even at less than 1 ppm. BMC Bioinformatics. 2006; 7:234. PMC: 1464138. DOI: 10.1186/1471-2105-7-234. View

Long R, Rowley D, Zamora E, Liu J, Bartlett D, Azam F . Antagonistic interactions among marine bacteria impede the proliferation of Vibrio cholerae. Appl Environ Microbiol. 2005; 71(12):8531-6. PMC: 1317384. DOI: 10.1128/AEM.71.12.8531-8536.2005. View

Hjelm M, Riaza A, Formoso F, Melchiorsen J, Gram L . Seasonal incidence of autochthonous antagonistic Roseobacter spp. and Vibrionaceae strains in a turbot larva (Scophthalmus maximus) rearing system. Appl Environ Microbiol. 2004; 70(12):7288-94. PMC: 535194. DOI: 10.1128/AEM.70.12.7288-7294.2004. View

Das P, Mukherjee S, Sen R . Antimicrobial potential of a lipopeptide biosurfactant derived from a marine Bacillus circulans. J Appl Microbiol. 2008; 104(6):1675-84. DOI: 10.1111/j.1365-2672.2007.03701.x. View

10.

Oliva B, ONeill A, Wilson J, OHanlon P, Chopra I . Antimicrobial properties and mode of action of the pyrrothine holomycin. Antimicrob Agents Chemother. 2001; 45(2):532-9. PMC: 90323. DOI: 10.1128/AAC.45.2.532-539.2001. View

11.

Ben-Haim Y, Thompson F, Thompson C, Cnockaert M, Hoste B, Swings J . Vibrio coralliilyticus sp. nov., a temperature-dependent pathogen of the coral Pocillopora damicornis. Int J Syst Evol Microbiol. 2003; 53(Pt 1):309-315. DOI: 10.1099/ijs.0.02402-0. View

12.

Fischbach M, Walsh C, Clardy J . The evolution of gene collectives: How natural selection drives chemical innovation. Proc Natl Acad Sci U S A. 2008; 105(12):4601-8. PMC: 2290807. DOI: 10.1073/pnas.0709132105. View

13.

Berdy J . Bioactive microbial metabolites. J Antibiot (Tokyo). 2005; 58(1):1-26. DOI: 10.1038/ja.2005.1. View

14.

Lang G, Mayhudin N, Mitova M, Sun L, van der Sar S, Blunt J . Evolving trends in the dereplication of natural product extracts: new methodology for rapid, small-scale investigation of natural product extracts. J Nat Prod. 2008; 71(9):1595-9. DOI: 10.1021/np8002222. View

15.

Nair S, Simidu U . Distribution and significance of heterotrophic marine bacteria with antibacterial activity. Appl Environ Microbiol. 1987; 53(12):2957-62. PMC: 204229. DOI: 10.1128/aem.53.12.2957-2962.1987. View

16.

Geng H, Bruhn J, Nielsen K, Gram L, Belas R . Genetic dissection of tropodithietic acid biosynthesis by marine roseobacters. Appl Environ Microbiol. 2008; 74(5):1535-45. PMC: 2258615. DOI: 10.1128/AEM.02339-07. View

17.

Gram L, Melchiorsen J, Bruhn J . Antibacterial activity of marine culturable bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms. Mar Biotechnol (NY). 2009; 12(4):439-51. DOI: 10.1007/s10126-009-9233-y. View

18.

Prado S, Romalde J, Montes J, Barja J . Pathogenic bacteria isolated from disease outbreaks in shellfish hatcheries. First description of Vibrio neptunius as an oyster pathogen. Dis Aquat Organ. 2006; 67(3):209-15. DOI: 10.3354/dao067209. View

19.

Fischbach M . Antibiotics from microbes: converging to kill. Curr Opin Microbiol. 2009; 12(5):520-7. PMC: 3176294. DOI: 10.1016/j.mib.2009.07.002. View

20.

Zhang L, An R, Wang J, Sun N, Zhang S, Hu J . Exploring novel bioactive compounds from marine microbes. Curr Opin Microbiol. 2005; 8(3):276-81. DOI: 10.1016/j.mib.2005.04.008. View