Applicability of RNA Standards for Evaluating RT-qPCR Assays and Platforms

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2011 Feb 22

PMID 21332979

Citations 19

Authors

Alison S Devonshire

Ramnath Elaswarapu

Carole A Foy

Affiliations

Soon will be listed here.

Abstract

The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.

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