HPTLC Densitometric Analysis of Arbutin in Bulk Drug and Methanolic Extracts of Arctostaphylos Uva-ursi

Overview

Journal Nat Prod Res

Specialty Biology

Date 2011 Feb 19

PMID 21331974

Citations 4

Authors

P Alam

S I Alqasoumi

F Shakeel

M S Abdel-Kader

Affiliations

Soon will be listed here.

Abstract

A high-performance thin layer chromatographic densitometric method for the analysis of arbutin was developed and validated in the present investigation. Arbutin was separated on aluminium-backed silica gel 60 F(254) plates with methanol : chloroform (3:7)% (v/v) as the mobile phase. This system was found to give a compact spot of arbutin at a retention factor (R(f)) value of 0.32 ± 0.02. The limit of detection and limit of quantification were found to be 35.42 and 106.26 ng/spot, respectively. The proposed method with a high degree of precision and accuracy was employed for the analysis of arbutin in the bulk drug and methanolic extract of Arctostaphylos uva-ursi.

Citing Articles

Preparation and stability investigation of ultrasound-assisted W/O/W multiple nanoemulsions co-loaded with hydrophobic curcumin and hydrophilic arbutin for tyrosinase inhibition.

Sainakham M, Promma B, Ngernthong A, Kiattisin K, Boonpisuttinant K, Wuttikul K Heliyon. 2024; 10(14):e34665.

PMID: 39130479 PMC: 11315199. DOI: 10.1016/j.heliyon.2024.e34665.

Anti-Toxoplasma In Vitro and In Vivo Activity of Pyrus boissieriana Arbutin-Rich Fraction.

Bahreini M, Pourmohammadi S, Gholami M, Habibollahi M, Pasdaran A, Hamedi A Acta Parasitol. 2024; 69(1):567-573.

PMID: 38231312 DOI: 10.1007/s11686-023-00759-x.

QUANTIFICATION OF GLYCYRRHIZIN BIOMARKER IN GLYCYRRHIZA GLABRA RHIZOME AND BABY HERBAL FORMULATIONS BY VALIDATED RP-HPTLC METHODS.

Alam P, Foudah A, Zaatout H, Y T K, Abdel-Kader M Afr J Tradit Complement Altern Med. 2017; 14(2):198-205.

PMID: 28573236 PMC: 5446444. DOI: 10.21010/ajtcam.v14i2.21.

Chromatographic finger print analysis of anti-inflammatory active extract fractions of aerial parts of Tribulus terrestris by HPTLC technique.

Mohammed M, Alajmi M, Alam P, Khalid H, Mahmoud A, Ahmed W Asian Pac J Trop Biomed. 2014; 4(3):203-8.

PMID: 25182438 PMC: 3868790. DOI: 10.1016/S2221-1691(14)60232-X.