Sertoli Cell-conditioned Medium Induces Germ Cell Differentiation in Human Embryonic Stem Cells

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2011 Feb 15

PMID 21318592

Citations 13

Authors

Mieke Geens

Karen Dora Sermon

Hilde Van de Velde

Herman Tournaye

Affiliations

Soon will be listed here.

Abstract

Purpose: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols.

Methods: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative quantification real-time RT-PCR to determine the efficiency of germ cell differentiation.

Results: VUB hESC lines can differentiate spontaneously towards the germ cell lineage, however, more consistently in an embryoid body approach than in monolayer cultures. BMPs and SCCM significantly improve VASA expression, but do not have a synergistic effect. Direct contact of differentiating hESC with Sertoli cells does not improve VASA expression.

Conclusions: SCCM contains inductive factors for germ cell differentiation and could represent an element for in-vitro differentiation to germ cells.

Citing Articles

Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells.

Rore H, Owen N, Pina-Aguilar R, Docherty K, Sekido R Commun Biol. 2021; 4(1):802.

PMID: 34183774 PMC: 8239049. DOI: 10.1038/s42003-021-02322-8.

Inducing Non-genetically Modified Induced Embryonic Sertoli Cells Derived From Embryonic Stem Cells With Recombinant Protein Factors.

Xu C, Mohsin A, Luo Y, Xie L, Peng Y, Wang Q Front Cell Dev Biol. 2021; 8:533543.

PMID: 33585437 PMC: 7875124. DOI: 10.3389/fcell.2020.533543.

Effect of Human Testicular Cells Conditioned Medium on Maturation and Morphology of Mouse Oocytes.

Adib M, Seifati S, Dehghani Ashkezari M, Akyash F, Khoradmehr A, Aflatoonian B Int J Fertil Steril. 2020; 14(3):175-184.

PMID: 33098383 PMC: 7604698. DOI: 10.22074/ijfs.2020.6097.

Human Pluripotent Stem Cells in Reproductive Science-a Comparison of Protocols Used to Generate and Define Male Germ Cells from Pluripotent Stem Cells.

Kurek M, Albalushi H, Hovatta O, Stukenborg J Int J Mol Sci. 2020; 21(3).

PMID: 32033159 PMC: 7038013. DOI: 10.3390/ijms21031028.

Indirect Co-Culture of Testicular Cells with Bone Marrow Mesenchymal Stem Cells Leads to Male Germ Cell-Specific Gene Expressions.

Ghorbanlou M, Abdanipour A, Shirazi R, Malekmohammadi N, Shokri S, Nejatbakhsh R Cell J. 2018; 20(4):505-512.

PMID: 30123996 PMC: 6099144. DOI: 10.22074/cellj.2019.5654.

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