Expression of VLA-4 Molecule in PBMC from Patients with Hemorrhagic Fever with Renal Syndrome

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2011 Feb 8

PMID 21298552

Authors

Ming Xie

Yanying Dong

Linjing He

Huixun Ren

Yuqiang Ji

Shemin Lv

Affiliations

Soon will be listed here.

Abstract

Objective And Design: The very late antigen-4 (VLA-4) bound to vascular cell adhesion molecule-1 could provide co-stimulatory signals for the activation of T lymphocytes, and these adhesion molecules play key roles in leukocyte adherence and propagation of inflammatory responses. We examined the levels of VLA-4 in the peripheral blood mononuclear cells (PBMC) of patients with hemorrhagic fever with renal syndrome (HFRS).

Materials And Methods: The levels of VLA-4 in PBMC samples collected from 53 patients by immunohistochemical staining were detected.

Results: The expression of VLA-4 in PBMC of HFRS patients at different stages were significantly higher than those in normal controls (P < 0.05), except recovery stage (P > 0.05). The expression of VLA-4 in PBMC of HFRS patients at different types were significantly higher than those in healthy controls (P < 0.05). The levels of VLA-4 in patients with HFRS were positively correlated with serum levels of blood urea nitrogen (BUN) and creatinine (Cr).

Conclusions: VLA-4 might play an important role in the immunopathological lesions of HFRS. We found that VLA-4 levels were closely correlated to the severity of the HFRS and the degree of kidney damage.

References

Muraro P, Leist T, Bielekova B, McFarland H . VLA-4/CD49d downregulated on primed T lymphocytes during interferon-beta therapy in multiple sclerosis. J Neuroimmunol. 2000; 111(1-2):186-94. DOI: 10.1016/s0165-5728(00)00362-3. View

Krakauer T, LeDuc J, Morrill J, Anderson A, KRAKAUER H . Serum levels of alpha and gamma interferons in hemorrhagic fever with renal syndrome. Viral Immunol. 1994; 7(2):97-101. DOI: 10.1089/vim.1994.7.97. View

Drew M, Barker H, Ball J, Pearson C, Cook G, Franklin I . Very late antigen (VLA) expression by normal and neoplastic human plasma cells; including an assessment of antibodies submitted to the Vth International Workshop on Leucocyte Differentiation Antigens using human myeloma cell lines. Leuk Res. 1996; 20(7):619-24. DOI: 10.1016/0145-2126(95)00097-6. View

Gavrilovskaya I, Brown E, Ginsberg M, Mackow E . Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins. J Virol. 1999; 73(5):3951-9. PMC: 104173. DOI: 10.1128/JVI.73.5.3951-3959.1999. View

Araki K, Yoshimatsu K, Lee B, Okumura M, Kariwa H, Takashima I . Age-dependent hantavirus-specific CD8(+) T-cell responses in mice infected with Hantaan virus. Arch Virol. 2004; 149(7):1373-82. DOI: 10.1007/s00705-003-0285-4. View

Mittelbrunn M, Molina A, Escribese M, Yanez-Mo M, Escudero E, Ursa A . VLA-4 integrin concentrates at the peripheral supramolecular activation complex of the immune synapse and drives T helper 1 responses. Proc Natl Acad Sci U S A. 2004; 101(30):11058-63. PMC: 503740. DOI: 10.1073/pnas.0307927101. View

Xiong L, Luo D, Zeng L, Li S . Determination of CD30 expression on peripheral blood T lymphocyte subsets in patients with hemorrhagic fever with renal syndrome by FCM. J Tongji Med Univ. 2003; 21(3):240-1. DOI: 10.1007/BF02886442. View

Ikeda Y, Fujimoto T, Ameno M, Shiiki H, Dohi K . Relationship between lupus nephritis activity and the serum level of soluble VCAM-1. Lupus. 1998; 7(5):347-54. DOI: 10.1191/096120398678920172. View

Fang L, Yan L, Liang S, de Vlas S, Feng D, Han X . Spatial analysis of hemorrhagic fever with renal syndrome in China. BMC Infect Dis. 2006; 6:77. PMC: 1471792. DOI: 10.1186/1471-2334-6-77. View

10.

Gai Z, Zhang Y, Dong G, Zu Y, Zhang Y, Wu S . [A clinical study on CD178 positive T lymphocyte in hemorrhagic fever with renal syndrome]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2006; 19(4):383-6. View

11.

Elangbam C, Qualls Jr C, Dahlgren R . Cell adhesion molecules--update. Vet Pathol. 1997; 34(1):61-73. DOI: 10.1177/030098589703400113. View

12.

Aplin A, Howe A, Alahari S, Juliano R . Signal transduction and signal modulation by cell adhesion receptors: the role of integrins, cadherins, immunoglobulin-cell adhesion molecules, and selectins. Pharmacol Rev. 1998; 50(2):197-263. View

13.

Zaki S, Greer P, Coffield L, Goldsmith C, Nolte K, Foucar K . Hantavirus pulmonary syndrome. Pathogenesis of an emerging infectious disease. Am J Pathol. 1995; 146(3):552-79. PMC: 1869168. View

14.

Yanagihara R, Silverman D . Experimental infection of human vascular endothelial cells by pathogenic and nonpathogenic hantaviruses. Arch Virol. 1990; 111(3-4):281-6. DOI: 10.1007/BF01311063. View

15.

Krakauer T, LeDuc J, KRAKAUER H . Serum levels of tumor necrosis factor-alpha, interleukin-1, and interleukin-6 in hemorrhagic fever with renal syndrome. Viral Immunol. 1995; 8(2):75-9. DOI: 10.1089/vim.1995.8.75. View

16.

Pensiero M, Sharefkin J, Dieffenbach C, Hay J . Hantaan virus infection of human endothelial cells. J Virol. 1992; 66(10):5929-36. PMC: 241470. DOI: 10.1128/JVI.66.10.5929-5936.1992. View

17.

Zheng F, Li L, Liu Z . [Immune-mediated tubulointerstitial injury in hemorrhagic fever with renal syndrome]. Zhonghua Yi Xue Za Zhi. 1996; 76(6):411-5. View

18.

Araki K, Yoshimatsu K, Lee B, Kariwa H, Takashima I, Arikawa J . A new model of Hantaan virus persistence in mice: the balance between HTNV infection and CD8(+) T-cell responses. Virology. 2004; 322(2):318-27. DOI: 10.1016/j.virol.2004.01.030. View

19.

Huang C, Jin B, Wang M, Li E, Sun C . Hemorrhagic fever with renal syndrome: relationship between pathogenesis and cellular immunity. J Infect Dis. 1994; 169(4):868-70. DOI: 10.1093/infdis/169.4.868. View

20.

Sundstrom J, McMullan L, Spiropoulou C, Hooper W, Ansari A, Peters C . Hantavirus infection induces the expression of RANTES and IP-10 without causing increased permeability in human lung microvascular endothelial cells. J Virol. 2001; 75(13):6070-85. PMC: 114323. DOI: 10.1128/JVI.75.13.6070-6085.2001. View