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Shuttle Vector System for Methanococcus Maripaludis with Improved Transformation Efficiency

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 2011 Feb 8
PMID 21296937
Citations 17
Authors
Alison D Walters
Sarah E Smith
James P J Chong
Affiliations
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Abstract

We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.

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