Hyaluronic Acid and Fibrin Hydrogels with Concentrated DNA/PEI Polyplexes for Local Gene Delivery

Overview

Journal J Control Release

Specialty Pharmacology

Date 2011 Feb 8

PMID 21295089

Citations 51

Authors

Yuguo Lei

Maha Rahim

Quinn Ng

Tatiana Segura

Affiliations

Soon will be listed here.

Abstract

Local delivery of DNA through a hydrogel scaffold would increase the applicability of gene therapy in tissue regeneration and cancer therapy. However, the delivery of DNA/cationic polymer nanoparticles (polyplexes) using hydrogels is challenging due to the aggregation and inactivation of polyplexes during their incorporation into hydrogel scaffolds. We developed a novel process (termed caged nanoparticle encapsulation or CnE) to load concentrated and unaggregated non-viral gene delivery nanoparticles into various hydrogels. Previously, we showed that PEG hydrogels loaded with DNA/PEI polyplexes through this process were able to deliver genes both in vitro and in vivo. In this study, we found that hyaluronic acid and fibrin hydrogels with concentrated and unaggregated polyplexes loaded through CnE were able to deliver genes in vivo as well, demonstrating the universality of the process.

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References

Brus C, Kleemann E, Aigner A, Czubayko F, Kissel T . Stabilization of oligonucleotide-polyethylenimine complexes by freeze-drying: physicochemical and biological characterization. J Control Release. 2004; 95(1):119-31. DOI: 10.1016/j.jconrel.2003.10.021. View

Jang J, Rives C, Shea L . Plasmid delivery in vivo from porous tissue-engineering scaffolds: transgene expression and cellular transfection. Mol Ther. 2005; 12(3):475-83. PMC: 2648405. DOI: 10.1016/j.ymthe.2005.03.036. View

Tyrone J, Mogford J, Chandler L, Ma C, Xia Y, Pierce G . Collagen-embedded platelet-derived growth factor DNA plasmid promotes wound healing in a dermal ulcer model. J Surg Res. 2000; 93(2):230-6. DOI: 10.1006/jsre.2000.5912. View

Krebs M, Jeon O, Alsberg E . Localized and sustained delivery of silencing RNA from macroscopic biopolymer hydrogels. J Am Chem Soc. 2009; 131(26):9204-6. DOI: 10.1021/ja9037615. View

Megeed Z, Haider M, Li D, OMalley Jr B, Cappello J, Ghandehari H . In vitro and in vivo evaluation of recombinant silk-elastinlike hydrogels for cancer gene therapy. J Control Release. 2004; 94(2-3):433-45. DOI: 10.1016/j.jconrel.2003.10.027. View

Bonadio J . Tissue engineering via local gene delivery: update and future prospects for enhancing the technology. Adv Drug Deliv Rev. 2000; 44(2-3):185-94. DOI: 10.1016/s0169-409x(00)00094-6. View

Kong H, Kim E, Huang Y, Mooney D . Design of biodegradable hydrogel for the local and sustained delivery of angiogenic plasmid DNA. Pharm Res. 2008; 25(5):1230-8. DOI: 10.1007/s11095-007-9526-7. View

Scherer F, Schillinger U, Putz U, Stemberger A, Plank C . Nonviral vector loaded collagen sponges for sustained gene delivery in vitro and in vivo. J Gene Med. 2002; 4(6):634-43. DOI: 10.1002/jgm.298. View

Allison S, Anchordoquy T . Mechanisms of protection of cationic lipid-DNA complexes during lyophilization. J Pharm Sci. 2000; 89(5):682-91. DOI: 10.1002/(SICI)1520-6017(200005)89:5<682::AID-JPS14>3.0.CO;2-#. View

10.

Quick D, Anseth K . DNA delivery from photocrosslinked PEG hydrogels: encapsulation efficiency, release profiles, and DNA quality. J Control Release. 2004; 96(2):341-51. DOI: 10.1016/j.jconrel.2004.01.021. View

11.

Ochiya T, Takahama Y, Nagahara S, Sumita Y, Hisada A, Itoh H . New delivery system for plasmid DNA in vivo using atelocollagen as a carrier material: the Minipellet. Nat Med. 1999; 5(6):707-10. DOI: 10.1038/9560. View

12.

Lechardeur D, Verkman A, Lukacs G . Intracellular routing of plasmid DNA during non-viral gene transfer. Adv Drug Deliv Rev. 2005; 57(5):755-67. DOI: 10.1016/j.addr.2004.12.008. View

13.

Bonadio J, Smiley E, Patil P, Goldstein S . Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration. Nat Med. 1999; 5(7):753-9. DOI: 10.1038/10473. View

14.

Moret I, Esteban Peris J, Guillem V, Benet M, Revert F, Dasi F . Stability of PEI-DNA and DOTAP-DNA complexes: effect of alkaline pH, heparin and serum. J Control Release. 2001; 76(1-2):169-81. DOI: 10.1016/s0168-3659(01)00415-1. View

15.

Trentin D, Hubbell J, Hall H . Non-viral gene delivery for local and controlled DNA release. J Control Release. 2005; 102(1):263-75. DOI: 10.1016/j.jconrel.2004.09.029. View

16.

Wieland J, Houchin-Ray T, Shea L . Non-viral vector delivery from PEG-hyaluronic acid hydrogels. J Control Release. 2007; 120(3):233-41. PMC: 2648399. DOI: 10.1016/j.jconrel.2007.04.015. View

17.

Trentin D, Hall H, Wechsler S, Hubbell J . Peptide-matrix-mediated gene transfer of an oxygen-insensitive hypoxia-inducible factor-1alpha variant for local induction of angiogenesis. Proc Natl Acad Sci U S A. 2006; 103(8):2506-11. PMC: 1413769. DOI: 10.1073/pnas.0505964102. View

18.

Kulkarni M, Breen A, Greiser U, OBrien T, Pandit A . Fibrin-lipoplex system for controlled topical delivery of multiple genes. Biomacromolecules. 2009; 10(6):1650-4. DOI: 10.1021/bm900248n. View

19.

Holladay C, Keeney M, Greiser U, Murphy M, OBrien T, Pandit A . A matrix reservoir for improved control of non-viral gene delivery. J Control Release. 2009; 136(3):220-5. DOI: 10.1016/j.jconrel.2009.02.006. View

20.

Cheema S, Chen E, Shea L, Mathur A . Regulation and guidance of cell behavior for tissue regeneration via the siRNA mechanism. Wound Repair Regen. 2007; 15(3):286-95. DOI: 10.1111/j.1524-475X.2007.00228.x. View