The Use of Some Nanoemulsions Based on Aqueous Propolis and Lycopene Extract in the Skin's Protective Mechanisms Against UVA Radiation

Overview

Journal J Nanobiotechnology

Publisher Biomed Central

Specialty Biotechnology

Date 2011 Feb 8

PMID 21294875

Citations 16

Authors

Monica V Butnariu

Camelia V Giuchici

Affiliations

Soon will be listed here.

Abstract

Background: The use of natural products based on aqueous extract of propolis and lycopene in the skin's protective mechanisms against UVA radiation was evaluated by means of experimental acute inflammation on rat paw edema. The aim of the present study was to evaluate the harmlessness of propolis - lycopene system through evaluation of skin level changes and anti-inflammatory action. The regenerative and protective effect of the aqueous propolis and lycopene extract is based on its richness in biologically active substances such as: tocopherols, flavonoids, amino acids, polyunsaturated fatty acids, the chlorophyll pigment, all substances with strong antioxidant activity, that modify the oxidative stress, mainly by reducing the prooxidant processes and enhancing the antioxidant ones. These substances participate in the synthesis of prostaglandins and phospholipids components of cell membrane thus enhancing skin protection mechanisms.

Results: The experimental systems offered a sustained release of the drug, in vitro, for aim eight hours. The prepared formulations aim did not reveal a deteriorating effect on tissues. They proved a better therapeutic efficiency Compared to standard suspension, they provided a better therapeutic efficiency coupled with extended time interval of tested parameters (24 hours). Preliminary examination of tissues showed that the experimental formulations did not irritate. Local application of propolis and lycopene aqueous extract nanoemulsion has a high potential both regarding its efficiency (the analgesic effect) and therapeutic safety.

Conclusions: This study demonstrates that propolis and lycopene extract nanoemulsions, preparations contains active substances, can confer better therapeutic effects than those of the conventional formulations, based on local control-release of dozed form, for a longer period of time, which probably improve its efficiency and skin acceptance, meaning a better compliance. The information obtained in the present study suggests that administration of propolis and lycopene aqueous extract nanoemulsion is safe. The preparation can be useful for further preclinical studies lycopene embedded in aqueous propolis extract to be used in pharmaceuticals (targeted medical therapy).

Citing Articles

Effects of Poly-L-Lactic Acid Fillers on Inflammatory Response and Collagen Synthesis in Different Animal Models.

He T, Zhang Z, Zhang X, Niu H, Wang S, Wang Q J Cosmet Dermatol. 2025; 24(2):e70000.

PMID: 39910771 PMC: 11799711. DOI: 10.1111/jocd.70000.

Molecular Characterization Reveals Biodiversity and Biopotential of Rhizobacterial Isolates of Bacillus Spp.

Sagar A, Rai S, Sharma S, Perveen K, Bukhari N, Sayyed R Microb Ecol. 2024; 87(1):83.

PMID: 38888737 PMC: 11189325. DOI: 10.1007/s00248-024-02397-w.

Encapsulation of propolis extracted with methylal in the chitosan nanoparticles and its antibacterial and cell cytotoxicity studies.

Vaseghi A, Parchin R, Chamanie K, Herb M, Maleki H, Sadeghizadeh M BMC Complement Med Ther. 2024; 24(1):165.

PMID: 38641781 PMC: 11027551. DOI: 10.1186/s12906-024-04472-8.

Skin Protection by Carotenoid Pigments.

Flieger J, Raszewska-Famielec M, Radzikowska-Buchner E, Flieger W Int J Mol Sci. 2024; 25(3).

PMID: 38338710 PMC: 10855854. DOI: 10.3390/ijms25031431.

Bioactive Compounds for Combating Oxidative Stress in Dermatology.

Turcov D, Zbranca-Toporas A, Suteu D Int J Mol Sci. 2023; 24(24).

PMID: 38139345 PMC: 10744063. DOI: 10.3390/ijms242417517.

References

Loftsson T, Masson M . Cyclodextrins in topical drug formulations: theory and practice. Int J Pharm. 2001; 225(1-2):15-30. DOI: 10.1016/s0378-5173(01)00761-x. View

Peak M, Peak J, Carnes B . Induction of direct and indirect single-strand breaks in human cell DNA by far- and near-ultraviolet radiations: action spectrum and mechanisms. Photochem Photobiol. 1987; 45(3):381-7. DOI: 10.1111/j.1751-1097.1987.tb05390.x. View

Cole C . Sunscreen protection in the ultraviolet A region: how to measure the effectiveness. Photodermatol Photoimmunol Photomed. 2001; 17(1):2-10. DOI: 10.1034/j.1600-0781.2001.017001002.x. View

Wang S, Setlow R, Berwick M, Polsky D, Marghoob A, Kopf A . Ultraviolet A and melanoma: a review. J Am Acad Dermatol. 2001; 44(5):837-46. DOI: 10.1067/mjd.2001.114594. View

Cunningham M, Johnson J, Giovanazzi S, Peak M . Photosensitized production of superoxide anion by monochromatic (290-405 nm) ultraviolet irradiation of NADH and NADPH coenzymes. Photochem Photobiol. 1985; 42(2):125-8. DOI: 10.1111/j.1751-1097.1985.tb01549.x. View

Blonska M, Bronikowska J, Pietsz G, Czuba Z, Scheller S, Krol W . Effects of ethanol extract of propolis (EEP) and its flavones on inducible gene expression in J774A.1 macrophages. J Ethnopharmacol. 2004; 91(1):25-30. DOI: 10.1016/j.jep.2003.11.011. View

Kumar A, Bagewadi A, Keluskar V, Singh M . Efficacy of lycopene in the management of oral submucous fibrosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007; 103(2):207-13. DOI: 10.1016/j.tripleo.2006.07.011. View

Riso P, Visioli F, Grande S, Guarnieri S, Gardana C, Simonetti P . Effect of a tomato-based drink on markers of inflammation, immunomodulation, and oxidative stress. J Agric Food Chem. 2006; 54(7):2563-6. DOI: 10.1021/jf053033c. View

Balasubramanian D . Ultraviolet radiation and cataract. J Ocul Pharmacol Ther. 2000; 16(3):285-97. DOI: 10.1089/jop.2000.16.285. View

10.

Shao A, Hathcock J . Risk assessment for the carotenoids lutein and lycopene. Regul Toxicol Pharmacol. 2006; 45(3):289-98. DOI: 10.1016/j.yrtph.2006.05.007. View

11.

Morliere P, Moysan A, Tirache I . Action spectrum for UV-induced lipid peroxidation in cultured human skin fibroblasts. Free Radic Biol Med. 1995; 19(3):365-71. DOI: 10.1016/0891-5849(95)00043-w. View

12.

Peak J, Pilas B, Dudek E, Peak M . DNA breaks caused by monochromatic 365 nm ultraviolet-A radiation or hydrogen peroxide and their repair in human epithelioid and xeroderma pigmentosum cells. Photochem Photobiol. 1991; 54(2):197-203. DOI: 10.1111/j.1751-1097.1991.tb02007.x. View

13.

Chandra R, Prabhuji M, Roopa D, Ravirajan S, Kishore H . Efficacy of lycopene in the treatment of gingivitis: a randomised, placebo-controlled clinical trial. Oral Health Prev Dent. 2008; 5(4):327-36. View

14.

Dudek E, Peak J, ROTH R, Peak M . Isolation of V79 fibroblast cell lines containing elevated metallothionein levels that have increased resistance to the cytotoxic effects of ultraviolet-A radiation. Photochem Photobiol. 1993; 58(6):836-40. DOI: 10.1111/j.1751-1097.1993.tb04980.x. View

15.

Chapple I . Reactive oxygen species and antioxidants in inflammatory diseases. J Clin Periodontol. 1997; 24(5):287-96. DOI: 10.1111/j.1600-051x.1997.tb00760.x. View

16.

Zigman S, McDaniel T, Schultz J, REDDAN J, Meydani M . Damage to cultured lens epithelial cells of squirrels and rabbits by UV-A (99.9%) plus UV-B (0.1%) radiation and alpha tocopherol protection. Mol Cell Biochem. 1995; 143(1):35-46. DOI: 10.1007/BF00925924. View

17.

Sesso H, Buring J, Norkus E, Gaziano J . Plasma lycopene, other carotenoids, and retinol and the risk of cardiovascular disease in men. Am J Clin Nutr. 2005; 81(5):990-7. DOI: 10.1093/ajcn/81.5.990. View

18.

Borrelli F, Maffia P, Pinto L, Ianaro A, Russo A, Capasso F . Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia. 2002; 73 Suppl 1:S53-63. DOI: 10.1016/s0367-326x(02)00191-0. View

19.

Kuusilehto A . Transmission of UVA radiation through epithelium of oral mucosa and skin in rat and man. Photodermatol Photoimmunol Photomed. 2000; 16(4):189-91. DOI: 10.1034/j.1600-0781.2000.160409.x. View

20.

Stocker R . Induction of haem oxygenase as a defence against oxidative stress. Free Radic Res Commun. 1990; 9(2):101-12. DOI: 10.3109/10715769009148577. View