Arachidonic Acid Stimulates Formation of a Novel Complex Containing Nucleolin and RhoA

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2011 Feb 2

PMID 21281639

Citations 11

Authors

Melissa C Garcia

Jason Williams

Katina Johnson

Kenneth Olden

John D Roberts

Affiliations

Soon will be listed here.

Abstract

Arachidonic acid (AA) stimulates cell adhesion through a p38 mitogen activated protein kinase-mediated RhoA signaling pathway. Here we report that a proteomic screen following AA-treatment identified nucleolin, a multifunctional nucleolar protein, in a complex with the GTPase, RhoA, that also included the Rho kinase, ROCK. AA-stimulated cell adhesion was inhibited by expression of nucleolin-targeted shRNA and formation of the multiprotein complex was blocked by expression of dominant-negative RhoA. AA-treatment also induced ROCK-dependent serine phosphorylation of nucleolin and translocation of nucleolin from the nucleus to the cytoplasm, where it appeared to co-localize with RhoA. These data suggest the existence of a new signaling pathway through which the location and post-translational state of nucleolin are modulated.

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