Isolation, in Vitro Maturation, and Fertilization of Germinal Vesicle Oocytes Obtained from the Intact Murine Ovary

Overview

Journal J In Vitro Fert Embryo Transf

Specialty Reproductive Medicine

Date 1990 Dec 1

PMID 2127601

Citations 2

Authors

G W Randall

S G Awadalla

C A SHIVERS

Affiliations

Soon will be listed here.

Abstract

The purpose of this investigation was to attempt to develop a process, utilizing a murine model, which would allow more efficient harvesting from the intact ovary and maturation in vitro of germinal vesicle (GV) oocytes. The recovery process yielded 25.5 +/- 4.5 (mean +/- SE) cumulus-free GV oocytes per animal. Treatment groups included culture medium (CM) supplemented with either estradiol (E2), follicle stimulating hormone (FSH), human chorionic gonadotropin (hCG), or prolactin (PRL). Among the hormone-free controls 83.2 +/- 1.6% of oocytes underwent GV breakdown, whereas 25.3 +/- 2.6% developed to the first polar body stage (PB-1) following 18 hr of incubation (n = 29 trials). Oocytes progressing to the PB-1 stage were inseminated in vitro. In vitro fertilization (IVF) of pooled in vitro matured (IVM) PB-1 oocytes (judged by two-cell formation) was 19.9%, which was significantly lower than in the group of in vivo matured oocytes (74.4%). E2 significantly increased the percentage of GV breakdown (control, 76.8 +/- 2.5%; E2 at 10 ng/ml, 92.9 +/- 2.5%, P less than 0.001; E2 at 100 ng/ml, 93.7 +/- 2.1%, P less than 0.001; and E2 at 1 micrograms/ml, 86.7 +/- 3.3%, P less than 0.05) but not PB-1 formation. Neither FSH nor hCG significantly increased GV breakdown or PB-1 formation.(ABSTRACT TRUNCATED AT 250 WORDS)

Citing Articles

Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts.

Cha S, Shin D, Kim B, Yoon S, Yoon T, Lee W Reprod Sci. 2014; 21(7):859-868.

PMID: 24401473 PMC: 4107563. DOI: 10.1177/1933719113518983.

Elevated serum progesterone values at the time of ovulation induction in luteal leuprolide acetate-down-regulated GIFT cycles are associated with decreased clinical pregnancy rates.

Randall G, Gantt P, Gantt D, Kirk M, Romines N J Assist Reprod Genet. 1996; 13(6):459-63.

PMID: 8835672 DOI: 10.1007/BF02066524.

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