Omega-3 Fatty Acid Supplementation in Cancer Therapy : Does Eicosapentanoic Acid Influence the Radiosensitivity of Tumor Cells?

Overview

Journal Strahlenther Onkol

Specialties Oncology
Radiology

Date 2011 Jan 27

PMID 21267532

Citations 4

Authors

Katrin Manda

Stephan Kriesen

Guido Hildebrandt

Rainer Fietkau

Gunther Klautke

Affiliations

Soon will be listed here.

Abstract

Purpose: The aim of this study was to evaluate whether the omega-3 polyunsaturated fatty acid cis-5,8,11,14,17-eicosapentanoic acid (EPA) can enhance the radiosensitivity of different human tumor cell lines.

Materials And Methods: Colon adenocarcinoma cells HT-29, and two glioblastoma multiforme tumor cells T98G and U251 were cultured under standard conditions. Cell growth was observed during administration with different concentrations of EPA, using it as the free fatty acid dissolved in ethanol or bound to bovine serum albumin. To investigate the influence of EPA (free and bound) on radiosensitivity, tumor cells were pretreated 30 minutes or 24 hours prior to irradiation with the fatty acid. Cell survival was measured by colony-forming assays.

Results: When combined with irradiation, incubation with EPA was found to result in enhanced radiosensitivity with substantial variation: while there was strong radiosensitization for HT-29 and U251 cells, almost no effect for T98G cells was observed. A marked radiosensitization was clearly dependent on the treatment schedule.

Conclusion: The observations suggest that EPA is not only a nutritional adjuvant but also may be a potential candidate to enhance the efficacy of irradiation on human cancer cells.

Citing Articles

The value of plasma omega-3 polyunsaturated fatty acids in predicting the response and prognosis of cervical squamous cell carcinoma patients to concurrent chemoradiotherapy.

Ping P, Li J, Xu X Front Pharmacol. 2024; 15:1379508.

PMID: 38860167 PMC: 11163051. DOI: 10.3389/fphar.2024.1379508.

FABP7 Facilitates Uptake of Docosahexaenoic Acid in Glioblastoma Neural Stem-like Cells.

Choi W, Xu X, Goruk S, Wang Y, Patel S, Chow M Nutrients. 2021; 13(8).

PMID: 34444824 PMC: 8402214. DOI: 10.3390/nu13082664.

Omega-3 Polyunsaturated Fatty Acids and Their Bioactive Metabolites in Gastrointestinal Malignancies Related to Unresolved Inflammation. A Review.

Irun P, Lanas A, Piazuelo E Front Pharmacol. 2019; 10:852.

PMID: 31427966 PMC: 6687876. DOI: 10.3389/fphar.2019.00852.

Radiosensitizing effect of epothilone B on human epithelial cancer cells.

Baumgart T, Klautke G, Kriesen S, Kuznetsov S, Weiss D, Fietkau R Strahlenther Onkol. 2012; 188(2):177-84.

PMID: 22234539 DOI: 10.1007/s00066-011-0029-4.

References

Argiles J . Cancer-associated malnutrition. Eur J Oncol Nurs. 2006; 9 Suppl 2:S39-50. DOI: 10.1016/j.ejon.2005.09.006. View

Milne G, Yin H, Morrow J . Human biochemistry of the isoprostane pathway. J Biol Chem. 2008; 283(23):15533-7. PMC: 2414280. DOI: 10.1074/jbc.R700047200. View

Mahoney E, Hamill A, Scott W, COHN Z . Response of endocytosis to altered fatty acyl composition of macrophage phospholipids. Proc Natl Acad Sci U S A. 1977; 74(11):4895-9. PMC: 432063. DOI: 10.1073/pnas.74.11.4895. View

von Schacky C . A review of omega-3 ethyl esters for cardiovascular prevention and treatment of increased blood triglyceride levels. Vasc Health Risk Manag. 2007; 2(3):251-62. PMC: 1993981. DOI: 10.2147/vhrm.2006.2.3.251. View

Chang C, Patel P, Kang N, Lawson J, Song W, Powell W . Eicosapentaenoic-acid-derived isoprostanes: synthesis and discovery of two major isoprostanes. Bioorg Med Chem Lett. 2008; 18(20):5523-7. PMC: 2581611. DOI: 10.1016/j.bmcl.2008.09.008. View

Das U . Gamma-linolenic acid therapy of human glioma-a review of in vitro, in vivo, and clinical studies. Med Sci Monit. 2007; 13(7):RA119-31. View

Calonghi N, Pagnotta E, Parolin C, Tognoli C, Boga C, Masotti L . 9-Hydroxystearic acid interferes with EGF signalling in a human colon adenocarcinoma. Biochem Biophys Res Commun. 2006; 342(2):585-8. DOI: 10.1016/j.bbrc.2006.02.008. View

Dittmann K, Mayer C, Rodemann H . Nuclear EGFR as novel therapeutic target: insights into nuclear translocation and function. Strahlenther Onkol. 2010; 186(1):1-6. DOI: 10.1007/s00066-009-2026-4. View

Ross J, Maingay J, Fearon K, Sangster K, Powell J . Eicosapentaenoic acid perturbs signalling via the NFkappaB transcriptional pathway in pancreatic tumour cells. Int J Oncol. 2003; 23(6):1733-8. View

10.

Colquhoun A, Schumacher R . gamma-Linolenic acid and eicosapentaenoic acid induce modifications in mitochondrial metabolism, reactive oxygen species generation, lipid peroxidation and apoptosis in Walker 256 rat carcinosarcoma cells. Biochim Biophys Acta. 2001; 1533(3):207-19. DOI: 10.1016/s1388-1981(01)00136-6. View

11.

Black H, Rhodes L . The potential of omega-3 fatty acids in the prevention of non-melanoma skin cancer. Cancer Detect Prev. 2006; 30(3):224-32. DOI: 10.1016/j.cdp.2006.04.008. View

12.

Chapkin R, McMurray D, Davidson L, Patil B, Fan Y, Lupton J . Bioactive dietary long-chain fatty acids: emerging mechanisms of action. Br J Nutr. 2008; 100(6):1152-7. PMC: 2648819. DOI: 10.1017/S0007114508992576. View

13.

Igarashi M, Miyazawa T . Do conjugated eicosapentaenoic acid and conjugated docosahexaenoic acid induce apoptosis via lipid peroxidation in cultured human tumor cells?. Biochem Biophys Res Commun. 2001; 270(2):649-56. DOI: 10.1006/bbrc.2000.2484. View

14.

Calonghi N, Pagnotta E, Parolin C, Molinari C, Boga C, Dal Piaz F . Modulation of apoptotic signalling by 9-hydroxystearic acid in osteosarcoma cells. Biochim Biophys Acta. 2007; 1771(2):139-46. DOI: 10.1016/j.bbalip.2006.11.012. View

15.

Marquardt F, Rodel F, Capalbo G, Weiss C, Rodel C . Molecular targeted treatment and radiation therapy for rectal cancer. Strahlenther Onkol. 2009; 185(6):371-8. DOI: 10.1007/s00066-009-1936-5. View

16.

Calonghi N, Cappadone C, Pagnotta E, Farruggia G, Buontempo F, Boga C . 9-Hydroxystearic acid upregulates p21(WAF1) in HT29 cancer cells. Biochem Biophys Res Commun. 2004; 314(1):138-42. DOI: 10.1016/j.bbrc.2003.12.066. View

17.

Gabrys D, Dorfler A, Yaromina A, Hessel F, Krause M, Oertel R . Effects of lovastatin alone or combined with irradiation on tumor cells in vitro and in vivo. Strahlenther Onkol. 2008; 184(1):48-53. DOI: 10.1007/s00066-008-1805-7. View

18.

Barzan D, Maier P, Jens Zeller W, Wenz F, Herskind C . Overexpression of caveolin-1 in lymphoblastoid TK6 cells enhances proliferation after irradiation with clinically relevant doses. Strahlenther Onkol. 2010; 186(2):99-106. DOI: 10.1007/s00066-010-2029-1. View

19.

Riediger N, Othman R, Suh M, Moghadasian M . A systemic review of the roles of n-3 fatty acids in health and disease. J Am Diet Assoc. 2009; 109(4):668-79. DOI: 10.1016/j.jada.2008.12.022. View

20.

Zhivotovsky B, Joseph B, Orrenius S . Tumor radiosensitivity and apoptosis. Exp Cell Res. 1999; 248(1):10-7. DOI: 10.1006/excr.1999.4452. View