HIV-1 Nef Increases Astrocyte Sensitivity Towards Exogenous Hydrogen Peroxide

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2011 Jan 25

PMID 21255447

Citations 13

Authors

Sabine Masanetz

Michael H Lehmann

Affiliations

Soon will be listed here.

Abstract

Background: HIV-1 infected individuals are under chronic exposure to reactive oxygen species (ROS) considered to be instrumental in the progression of AIDS and the development of HIV-1 associated dementia (HAD). Astrocytes support neuronal function and protect them against cytotoxic substances including ROS. The protein HIV-1 Nef, a progression factor in AIDS pathology is abundantly expressed in astrocytes in patients with HAD, and thus may influence its functions.

Results: Endogenous expressed HIV-1 Nef leads to increased sensitivity of human astrocytes towards exogenous hydrogen peroxide but not towards TNF-alpha. Cell death of nef-expressing astrocytes exposed to 10 μM hydrogen peroxide for 30 min occurred within 4 h.

Conclusion: HIV-1 Nef may contribute to neuronal dysfunction and the development of HAD by causing death of astrocytes through decreasing their tolerance for hydrogen peroxide.

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