Testing for Chlamydia Trachomatis

Overview

Journal Can Fam Physician

Specialties General Medicine
Public Health

Date 2011 Jan 22

PMID 21253058

Authors

J E Graham

Affiliations

Soon will be listed here.

Abstract

Chlamydia trachomatis is a highly prevalent and damaging sexually transmitted disease. Since it may occur in an asymptomatic state, it is important for family physicians to know when screening or testing for this infection is indicated. The value of screening may be increased by correct collection, storage and transport of specimens. The techniques currently used in the laboratory to test specimens for chlamydia include cell culture, ELISA and immunofluorescent antibody (IFA) assay. Cell culture remains the 'gold standard'. The IFA and ELISA are simpler and quicker to perform than is culture and have sufficiently comparable measurements of accuracy.

References

Chernesky M, Mahony J, Castriciano S, Mores M, STEWART I, Landis S . Detection of Chlamydia trachomatis antigens by enzyme immunoassay and immunofluorescence in genital specimens from symptomatic and asymptomatic men and women. J Infect Dis. 1986; 154(1):141-8. DOI: 10.1093/infdis/154.1.141. View

Williams T, MANIAR A, Brunham R, Hammond G . Identification of Chlamydia trachomatis by direct immunofluorescence applied in specimens originating in remote areas. J Clin Microbiol. 1985; 22(6):1053-4. PMC: 271878. DOI: 10.1128/jcm.22.6.1053-1054.1985. View

Nettleman M, Jones R, Roberts S, Katz B, Washington A, Dittus R . Cost-effectiveness of culturing for Chlamydia trachomatis. A study in a clinic for sexually transmitted diseases. Ann Intern Med. 1986; 105(2):189-96. DOI: 10.7326/0003-4819-105-2-189. View

Jones R, Katz B, Van Der Pol B, Caine V, Batteiger B, Newhall W . Effect of blind passage and multiple sampling on recovery of Chlamydia trachomatis from urogenital specimens. J Clin Microbiol. 1986; 24(6):1029-33. PMC: 269092. DOI: 10.1128/jcm.24.6.1029-1033.1986. View

Schachter J . Sexually transmitted chlamydia trachomatis infection: management of the most common venereal disease. Postgrad Med. 1982; 72(4):60-2, 64, 67 passim. DOI: 10.1080/00325481.1982.11716212. View

HOWARD L, COLEMAN P, England B, Herrmann J . Evaluation of chlamydiazyme for the detection of genital infections caused by Chlamydia trachomatis. J Clin Microbiol. 1986; 23(2):329-32. PMC: 268636. DOI: 10.1128/jcm.23.2.329-332.1986. View

Morgan-Capner P, Hudson P, Cansfield J, Saeed A . Detection of Chlamydia trachomatis by enzyme immunoassay, immunofluorescence, and cell culture. J Clin Pathol. 1986; 39(2):232. PMC: 499690. DOI: 10.1136/jcp.39.2.232-a. View

Thomas B, Evans R, Hawkins D, Taylor-Robinson D . Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation. J Clin Pathol. 1984; 37(7):812-6. PMC: 498816. DOI: 10.1136/jcp.37.7.812. View

THOMPSON S, Washington A . Epidemiology of sexually transmitted Chlamydia trachomatis infections. Epidemiol Rev. 1983; 5:96-123. DOI: 10.1093/oxfordjournals.epirev.a036266. View

10.

Podgore J, Holmes K, ALEXANDER E . Asymptomatic urethral infections due to Chlamydia trachomatis in male U.S. military personnel. J Infect Dis. 1982; 146(6):828. DOI: 10.1093/infdis/146.6.828. View

11.

Singal S, Reichman R, Graman P, Greisberger C, Trupei M, Menegus M . Isolation of Chlamydia trachomatis from men with urethritis: relative value of one vs. two swabs and influence of concomitant gonococcal infection. Sex Transm Dis. 1986; 13(1):50-2. DOI: 10.1097/00007435-198601000-00011. View

12.

Kiviat N, Wolner-Hanssen P, Peterson M, Wasserheit J, Stamm W, Eschenbach D . Localization of Chlamydia trachomatis infection by direct immunofluorescence and culture in pelvic inflammatory disease. Am J Obstet Gynecol. 1986; 154(4):865-73. DOI: 10.1016/0002-9378(86)90473-4. View

13.

Tjiam K, van Heijst B, van Zuuren A, Wagenvoort J, van Joost T, Stolz E . Evaluation of an enzyme immunoassay for the diagnosis of chlamydial infections in urogenital specimens. J Clin Microbiol. 1986; 23(4):752-4. PMC: 362830. DOI: 10.1128/jcm.23.4.752-754.1986. View

14.

Forbes B, Bartholoma N, McMillan J, Roefaro M, Weiner L, Welych L . Evaluation of a monoclonal antibody test to detect chlamydia in cervical and urethral specimens. J Clin Microbiol. 1986; 23(6):1136-7. PMC: 268809. DOI: 10.1128/jcm.23.6.1136-1137.1986. View

15.

Amortegui A, Meyer M . Enzyme immunoassay for detection of Chlamydia trachomatis from the cervix. Obstet Gynecol. 1985; 65(4):523-6. View

16.

Hallsworth P, McDonald P . Detection of Chlamydia trachomatis with fluorescent monoclonal antibody. Pathology. 1985; 17(3):500-2. DOI: 10.3109/00313028509105509. View

17.

Shafer M, Vaughan E, Lipkin E, Moscicki B, Schachter J . Evaluation of fluorescein-conjugated monoclonal antibody test to detect Chlamydia trachomatis endocervical infections in adolescent girls. J Pediatr. 1986; 108(5 Pt 1):779-83. DOI: 10.1016/s0022-3476(86)81068-x. View

18.

Coudron P, Fedorko D, Dawson M, Kaplowitz L, Brookman R, Dalton H . Detection of Chlamydia trachomatis in genital specimens by the Microtrak direct specimen test. Am J Clin Pathol. 1986; 85(1):89-92. DOI: 10.1093/ajcp/85.1.89. View

19.

Embil J, Thiebaux H, Manuel F, Pereira L, MacDonald S . Sequential cervical specimens and the isolation of Chlamydia trachomatis: factors affecting detection. Sex Transm Dis. 1983; 10(2):62-6. DOI: 10.1097/00007435-198304000-00003. View

20.

Mohanty K, ONeill J, HAMBLING M . Comparison of enzyme immunoassays and cell culture for detecting Chlamydia trachomatis. Genitourin Med. 1986; 62(3):175-6. PMC: 1011931. DOI: 10.1136/sti.62.3.175. View