Increased Detection of Clinically Significant Antibodies and Decreased Incidence of Delayed Haemolytic Transfusion Reaction with the Indirect Antiglobulin Test Potentiated by Polyethylene Glycol Compared to Albumin: a Japanese Study

Overview

Journal Blood Transfus

Specialty Hematology

Date 2011 Jan 22

PMID 21251459

Citations 2

Authors

Miho Okutsu

Hitoshi Ohto

Hiroyasu Yasuda

Kinuyo Kawabata

Satoshi Ono

Shunnichi Saito

Akiko Sugawara

Masami Kikuchi

Saori Miura

Youko Ishii

Kazuya Watanabe

Yuriko Tohyama

Kenneth E Nollet

Affiliations

Soon will be listed here.

Abstract

Background: The indirect antiglobulin test (IAT) can be potentiated by agents such as polyethylene glycol (PEG-IAT) and albumin (Alb-IAT). PEG-IAT is generally regarded as superior to Alb-IAT for the detection of clinically significant red blood cell (RBC) antibodies. However, supporting data come from Caucasian-dominant populations. Non-Caucasian populations should be investigated as well.

Material And Methods: In this single-centre, retrospective, sequential study, Alb-IAT was used from 1989 to 1996 (8 years) and PEG-IAT from 1997 to 2008 (12 years). Pre-transfusion RBC alloantibody detection rates and specificity, post-transfusion alloantibody production, and the incidence of delayed haemolytic transfusion reaction were assessed and compared for the two periods.

Results: Although overall RBC alloantibody detection rates were comparable, PEG-IAT more frequently detected clinically significant antibodies such as anti-E, anti-Fy(b), and anti-Jk(a), and less frequently detected insignificant antibodies such as anti-Le(b) and anti-P(1). New alloantibodies emerged comparably during the two periods. Delayed haemolytic transfusion reaction was less frequent during the PEG-IAT period (0.30% versus 0.12%, p<0.05).

Conclusion: PEG-IAT was superior in the detection of clinically significant antibodies, reduced the detection of insignificant antibodies, and prevented delayed haemolytic transfusion reaction better than Alb-IAT among Japanese transfusion recipients in this retrospective survey of limited power.

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