Replacement of the 3' Untranslated Variable Region of Mosquito-borne Dengue Virus with That of Tick-borne Langat Virus Does Not Alter Vector Specificity

Overview

Journal J Gen Virol

Specialty Microbiology

Date 2011 Jan 11

PMID 21216984

Citations 11

Authors

Ebenezer Tumban

Dana N Mitzel

Nyree E Maes

Christopher T Hanson

Stephen S Whitehead

Kathryn A Hanley

Affiliations

Soon will be listed here.

Abstract

The four major flavivirus clades are transmitted by mosquitoes, ticks, directly between vertebrates or directly between arthropods, respectively, but the molecular determinants of mode of transmission in flaviviruses are unknown. To assess the role of the UTRs in transmission, we generated chimeric genomes in which the 5' UTR, capsid and/or 3' UTR of mosquito-borne dengue virus serotype 4 (rDENV-4) were replaced, separately or in combination, with those of tick-borne Langat virus (rLGTV). None of the chimeric genomes yielded detectable virus following transfection. Replacement of the variable region (VR) in the rDENV-4 3' UTR with that of rLGTV generated virus rDENV-4-rLGTswapVR, which showed lower replication than its wild-type parents in mammalian but not mosquito cells in culture and was able to infect mosquitoes in vivo. Neither rDENV-4 nor rDENV-4-rLGTswapVR could infect larval Ixodes scapularis ticks immersed in virus, while rLGTV was highly infectious via this route.

Citing Articles

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