Structure of Precursor-bound NifEN: a Nitrogenase FeMo Cofactor Maturase/insertase

Overview

Journal Science

Specialty Science

Date 2011 Jan 8

PMID 21212358

Citations 52

Authors

Jens T Kaiser

Yilin Hu

Jared A Wiig

Douglas C Rees

Markus W Ribbe

Affiliations

Soon will be listed here.

Abstract

NifEN plays an essential role in the biosynthesis of the nitrogenase iron-molybdenum (FeMo) cofactor (M cluster). It is an α(2)β(2) tetramer that is homologous to the catalytic molybdenum-iron (MoFe) protein (NifDK) component of nitrogenase. NifEN serves as a scaffold for the conversion of an iron-only precursor to a matured form of the M cluster before delivering the latter to its target location within NifDK. Here, we present the structure of the precursor-bound NifEN of Azotobacter vinelandii at 2.6 angstrom resolution. From a structural comparison of NifEN with des-M-cluster NifDK and holo NifDK, we propose similar pathways of cluster insertion for the homologous NifEN and NifDK proteins.

Citing Articles

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