Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis

Overview

Journal Biochem Res Int

Publisher Wiley

Specialty Biochemistry

Date 2010 Dec 29

PMID 21188078

Citations 4

Authors

Nagaraja N Nagre

Vishwanath B Chachadi

Sachin M Eligar

C Shubhada

Radha Pujari

Padma Shastry

Bale M Swamy

Shashikala R Inamdar

Affiliations

Soon will be listed here.

Abstract

Ophthalmic mycoses caused by infectious fungi are being recognized as a serious concern since they lead to total blindness. Cephalosporium is one amongst several opportunistic fungal species implicated in ophthalmic infections leading to mycotic keratitis. A mitogenic lectin has been purified from the mycelia of fungus Cephalosporium, isolated from the corneal smears of a keratitis patient. Cephalosporium lectin (CSL) is a tetramer with subunit mass of 14 kDa, agglutinates human A, B, and O erythrocytes, and exhibits high affinity for mucin compared to fetuin and asialofetuin but does not bind to simple sugars indicating its complex sugar specificity. CSL showed strong binding to normal human peripheral blood mononuclear cells (PBMCs) to elicit mitogenic activity. The sugar specificity of the lectin and its interaction with PBMCs to exhibit mitogenic effect indicate its possible role in adhesion and infection process of Cephalosporium.

Citing Articles

Purification, characterization of an entomopathogenic fungal lectin from Purpureocillium lilacinum and its involvement in pathogenesis leading to mycotic keratitis.

Jagadeesh N, Kulkani S, Chachadi V, Roy S, Inamdar S Arch Microbiol. 2022; 204(11):673.

PMID: 36255493 DOI: 10.1007/s00203-022-03243-8.

An overview of lectin-glycan interactions: a key event in initiating fungal infection and pathogenesis.

Ballal S, Inamdar S Arch Microbiol. 2018; 200(3):371-382.

PMID: 29435599 DOI: 10.1007/s00203-018-1487-1.

Mitogenic lectins from Cephalosporium curvulum (CSL) and Aspergillus oryzae (AOL) mediate host-pathogen interactions leading to mycotic keratitis.

Ballal S, Belur S, Laha P, Roy S, Swamy B, Inamdar S Mol Cell Biochem. 2017; 434(1-2):209-219.

PMID: 28470344 DOI: 10.1007/s11010-017-3050-9.

Exquisite specificity of mitogenic lectin from Cephalosporium curvulum to core fucosylated N-glycans.

Inamdar S, Eligar S, Ballal S, Belur S, Kalraiya R, Swamy B Glycoconj J. 2015; 33(1):19-28.

PMID: 26514868 DOI: 10.1007/s10719-015-9628-0.

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