AIK-C Measles Vaccine Expressing Fusion Protein of Respiratory Syncytial Virus Induces Protective Antibodies in Cotton Rats

Overview

Journal Vaccine

Specialty Allergy & Immunology

Date 2010 Dec 28

PMID 21185852

Citations 13

Authors

Akihito Sawada

Katsuhiro Komase

Tetsuo Nakayama

Affiliations

Soon will be listed here.

Abstract

Respiratory syncytial virus (RSV) is the most common cause of respiratory infection in infants, and no vaccine is available. In this report, recombinant AIK-C measles vaccines, expressing the RSV G or F protein of subgroup A (MVAIK/RSV/G or F), were investigated as a RSV vaccine candidate. MVAIK/RSV/G or F had the original ts phenotype and expressed RSV/G or F protein. Cross-reactive neutralizing antibodies against RSV subgroups A and B were detected in cotton rats immunized intramuscularly with MVAIK/RSV/F but not MVAIK/RSV/G. In cotton rats infected with RSV, RSV was recovered and lung histopathological finding was compatible with interstitial pneumonia, demonstrating thickening of alveolar walls and infiltration of mononuclear cells. When cotton rats immunized with MVAIK/RSV/F were challenged with homologous RSV subgroup A, no infectious RSV was recovered and very mild inflammation was noted without RSV antigen expression. When they were challenged with subgroup B, protective efficacy decreased. When cotton rats immunized with MVAIK/RSV/G were challenged with RSV subgroup A, low levels of infectious virus were recovered from lung. When challenged with subgroup B, no protective effects was demonstrated, demonstrating large amounts of RSV antigen in bronchial-epithelial cells. MVAIK/RSV/F is promising candidate and protective effects should be confirmed in monkey model.

Citing Articles

Versatility of live-attenuated measles viruses as platform technology for recombinant vaccines.

Ebenig A, Lange M, Muhlebach M NPJ Vaccines. 2022; 7(1):119.

PMID: 36243743 PMC: 9568972. DOI: 10.1038/s41541-022-00543-4.

Chimeric Measles Virus (MV/RSV), Having Ectodomains of Respiratory Syncytial Virus (RSV) F and G Proteins Instead of Measles Envelope Proteins, Induced Protective Antibodies against RSV.

Sawada A, Ito T, Yamaji Y, Nakayama T Vaccines (Basel). 2021; 9(2).

PMID: 33669275 PMC: 7920054. DOI: 10.3390/vaccines9020156.

Recombinant Measles AIK-C Vaccine Strain Expressing Influenza HA Protein.

Ito T, Kumagai T, Yamaji Y, Sawada A, Nakayama T Vaccines (Basel). 2020; 8(2).

PMID: 32230902 PMC: 7349030. DOI: 10.3390/vaccines8020149.

Simultaneous Administration of Recombinant Measles Viruses Expressing Respiratory Syncytial Virus Fusion (F) and Nucleo (N) Proteins Induced Humoral and Cellular Immune Responses in Cotton Rats.

Yamaji Y, Sawada A, Yasui Y, Ito T, Nakayama T Vaccines (Basel). 2019; 7(1).

PMID: 30836661 PMC: 6466305. DOI: 10.3390/vaccines7010027.

Vaccine platform recombinant measles virus.

Muhlebach M Virus Genes. 2017; 53(5):733-740.

PMID: 28710608 PMC: 7089060. DOI: 10.1007/s11262-017-1486-3.

References

Despres P, Combredet C, Frenkiel M, Lorin C, Brahic M, Tangy F . Live measles vaccine expressing the secreted form of the West Nile virus envelope glycoprotein protects against West Nile virus encephalitis. J Infect Dis. 2004; 191(2):207-14. DOI: 10.1086/426824. View

Makino S, Sasaki K, Nakamura N, Nakagawa M, Nakajima S . Studies on the modification of the live AIK measles vaccine. II. Development and evaluation of the live AIK-C measles vaccine. Kitasato Arch Exp Med. 1974; 47(1-2):13-21. View

CHANOCK R, FINBERG L . Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). II. Epidemiologic aspects of infection in infants and young children. Am J Hyg. 1957; 66(3):291-300. DOI: 10.1093/oxfordjournals.aje.a119902. View

Polack F, Teng M, Collins P, Prince G, Exner M, Regele H . A role for immune complexes in enhanced respiratory syncytial virus disease. J Exp Med. 2002; 196(6):859-65. PMC: 2194058. DOI: 10.1084/jem.20020781. View

Collins P, Hill M, Camargo E, Grosfeld H, Chanock R, Murphy B . Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for.... Proc Natl Acad Sci U S A. 1995; 92(25):11563-7. PMC: 40442. DOI: 10.1073/pnas.92.25.11563. View

Whitehead S, Firestone C, Karron R, Crowe Jr J, Elkins W, Collins P . Addition of a missense mutation present in the L gene of respiratory syncytial virus (RSV) cpts530/1030 to RSV vaccine candidate cpts248/404 increases its attenuation and temperature sensitivity. J Virol. 1999; 73(2):871-7. PMC: 103906. DOI: 10.1128/JVI.73.2.871-877.1999. View

Lofland J, OConnor J, Chatterton M, Moxey E, PADDOCK L, Nash D . Palivizumab for respiratory syncytial virus prophylaxis in high-risk infants: a cost-effectiveness analysis. Clin Ther. 2000; 22(11):1357-69. DOI: 10.1016/s0149-2918(00)83032-5. View

Gonzalez P, Bueno S, Riedel C, Kalergis A . Impairment of T cell immunity by the respiratory syncytial virus: targeting virulence mechanisms for therapy and prophylaxis. Curr Med Chem. 2009; 16(34):4609-25. DOI: 10.2174/092986709789760724. View

Karron R, Wright P, Crowe Jr J, Thompson J, Makhene M, Casey R . Evaluation of two live, cold-passaged, temperature-sensitive respiratory syncytial virus vaccines in chimpanzees and in human adults, infants, and children. J Infect Dis. 1997; 176(6):1428-36. DOI: 10.1086/514138. View

10.

Anderson L, Hierholzer J, Tsou C, Hendry R, Fernie B, Stone Y . Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. J Infect Dis. 1985; 151(4):626-33. DOI: 10.1093/infdis/151.4.626. View

11.

Boukhvalova M, Prince G, Blanco J . The cotton rat model of respiratory viral infections. Biologicals. 2009; 37(3):152-9. PMC: 2882635. DOI: 10.1016/j.biologicals.2009.02.017. View

12.

Piedra P, Cron S, Jewell A, Hamblett N, McBride R, Palacio M . Immunogenicity of a new purified fusion protein vaccine to respiratory syncytial virus: a multi-center trial in children with cystic fibrosis. Vaccine. 2003; 21(19-20):2448-60. DOI: 10.1016/s0264-410x(03)00098-7. View

13.

Liniger M, Zuniga A, Tamin A, Azzouz-Morin T, Knuchel M, Marty R . Induction of neutralising antibodies and cellular immune responses against SARS coronavirus by recombinant measles viruses. Vaccine. 2008; 26(17):2164-74. PMC: 7115634. DOI: 10.1016/j.vaccine.2008.01.057. View

14.

Teng M, Whitehead S, Bermingham A, St Claire M, Elkins W, Murphy B . Recombinant respiratory syncytial virus that does not express the NS1 or M2-2 protein is highly attenuated and immunogenic in chimpanzees. J Virol. 2000; 74(19):9317-21. PMC: 102132. DOI: 10.1128/jvi.74.19.9317-9321.2000. View

15.

Wild T, Buckland R . Functional aspects of envelope-associated measles virus proteins. Curr Top Microbiol Immunol. 1995; 191:51-64. DOI: 10.1007/978-3-642-78621-1_4. View

16.

Becker Y . Respiratory syncytial virus (RSV) evades the human adaptive immune system by skewing the Th1/Th2 cytokine balance toward increased levels of Th2 cytokines and IgE, markers of allergy--a review. Virus Genes. 2006; 33(2):235-52. DOI: 10.1007/s11262-006-0064-x. View

17.

Nakayama T, Onoda K . Vaccine adverse events reported in post-marketing study of the Kitasato Institute from 1994 to 2004. Vaccine. 2006; 25(3):570-6. DOI: 10.1016/j.vaccine.2006.05.130. View

18.

Rima B, Earle J, Yeo R, Herlihy L, Baczko K, Ter Meulen V . Temporal and geographical distribution of measles virus genotypes. J Gen Virol. 1995; 76 ( Pt 5):1173-80. DOI: 10.1099/0022-1317-76-5-1173. View

19.

De Waal L, Wyatt L, Yuksel S, van Amerongen G, Moss B, Niesters H . Vaccination of infant macaques with a recombinant modified vaccinia virus Ankara expressing the respiratory syncytial virus F and G genes does not predispose for immunopathology. Vaccine. 2004; 22(8):923-6. DOI: 10.1016/j.vaccine.2003.10.010. View

20.

Ebbert J, Limper A . Respiratory syncytial virus pneumonitis in immunocompromised adults: clinical features and outcome. Respiration. 2005; 72(3):263-9. DOI: 10.1159/000085367. View