Genotypic Differences in Al Resistance and the Role of Cell-wall Pectin in Al Exclusion from the Root Apex in Fagopyrum Tataricum

Overview

Journal Ann Bot

Publisher Oxford University Press

Specialty Biology

Date 2010 Dec 25

PMID 21183454

Citations 19

Authors

Jian Li Yang

Xiao Fang Zhu

Cheng Zheng

Yue Jiao Zhang

Shao Jian Zheng

Affiliations

Soon will be listed here.

Abstract

Background And Aims: Aluminium (Al) toxicity is one of the factors limiting crop production on acid soils. However, genotypic differences exist among plant species or cultivars in response to Al toxicity. This study aims to investigate genotypic differences among eight cultivars of tatary buckwheat (Fagopyrum tataricum) for Al resistance and explore the possible mechanisms of Al resistance.

Methods: Al resistance was evaluated based on relative root elongation (root elongation with Al/root elongation without Al). Root apex Al content, pectin content and exudation of root organic acids were determined and compared.

Key Results: Genotypic differences among the eight cultivars were correlated with exclusion of Al from the root apex. However, there was a lack of correlation between Al exclusion and Al-induced oxalate secretion. Interestingly, cell-wall pectin content of the root apex was generally lower in Al-resistant cultivars than in Al-sensitive cultivars. Although we were unable to establish a significant correlation between Al exclusion and pectin content among the eight cultivars, a strong correlation could be established among six cultivars, in which the pectin content in the most Al-resistant cultivar 'Chuan' was significantly lower than that in the most Al-sensitive cultivar 'Liuku2'. Furthermore, root apex cell-wall pectin methylesterase activity (PME) was similar in 'Chuan' and 'Liuku2' in the absence of Al, but Al treatment resulted in increased PME activity in 'Liuku2' compared with 'Chuan'. Immunolocalization of pectins also showed that the two cultivars had similar amounts of either low-methyl-ester pectins or high-methyl-ester pectins in the absence of Al, but Al treatment resulted in a more significant increase of low-methyl-ester pectins and decrease of high-methyl-ester pectins in 'Liuku2'.

Conclusions: Cell-wall pectin content may contribute, at least in part, to differential Al resistance among tatary buckwheat cultivars.

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References

Ezaki B, Gardner R, Ezaki Y, Matsumoto H . Expression of aluminum-induced genes in transgenic arabidopsis plants can ameliorate aluminum stress and/or oxidative stress. Plant Physiol. 2000; 122(3):657-65. PMC: 58900. DOI: 10.1104/pp.122.3.657. View

Kochian L, Hoekenga O, Pineros M . How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorous efficiency. Annu Rev Plant Biol. 2004; 55:459-93. DOI: 10.1146/annurev.arplant.55.031903.141655. View

Zheng S, Yang J, He Y, Yu X, Zhang L, You J . Immobilization of aluminum with phosphorus in roots is associated with high aluminum resistance in buckwheat. Plant Physiol. 2005; 138(1):297-303. PMC: 1104184. DOI: 10.1104/pp.105.059667. View

Horst W, Wang Y, Eticha D . The role of the root apoplast in aluminium-induced inhibition of root elongation and in aluminium resistance of plants: a review. Ann Bot. 2010; 106(1):185-97. PMC: 2889789. DOI: 10.1093/aob/mcq053. View

Macdonald T, Martin R . Aluminum ion in biological systems. Trends Biochem Sci. 1988; 13(1):15-9. DOI: 10.1016/0968-0004(88)90012-6. View

Famoso A, Clark R, Shaff J, Craft E, McCouch S, Kochian L . Development of a novel aluminum tolerance phenotyping platform used for comparisons of cereal aluminum tolerance and investigations into rice aluminum tolerance mechanisms. Plant Physiol. 2010; 153(4):1678-91. PMC: 2923895. DOI: 10.1104/pp.110.156794. View

Taylor G, Hunter D, Bertsch P, Elmore D, Rengel Z, Reid R . Direct measurement of aluminum uptake and distribution in single cells of Chara corallina. Plant Physiol. 2000; 123(3):987-96. PMC: 59061. DOI: 10.1104/pp.123.3.987. View

Tabuchi A, Matsumoto H . Changes in cell-wall properties of wheat (Triticum aestivum) roots during aluminum-induced growth inhibition. Physiol Plant. 2001; 112(3):353-358. DOI: 10.1034/j.1399-3054.2001.1120308.x. View

Anthon G, Barrett D . Comparison of three colorimetric reagents in the determination of methanol with alcohol oxidase. Application to the assay of pectin methylesterase. J Agric Food Chem. 2004; 52(12):3749-53. DOI: 10.1021/jf035284w. View

10.

Delhaize E, Gruber B, Ryan P . The roles of organic anion permeases in aluminium resistance and mineral nutrition. FEBS Lett. 2007; 581(12):2255-62. DOI: 10.1016/j.febslet.2007.03.057. View

11.

Ma J, Shen R, Nagao S, Tanimoto E . Aluminum targets elongating cells by reducing cell wall extensibility in wheat roots. Plant Cell Physiol. 2004; 45(5):583-9. DOI: 10.1093/pcp/pch060. View

12.

Jian Zheng S , Feng Ma J , Matsumoto . High aluminum resistance in buckwheat. I. Al-induced specific secretion of oxalic acid from root tips . Plant Physiol. 1998; 117(3):745-51. PMC: 34929. DOI: 10.1104/pp.117.3.745. View

13.

Wenzl P, Patino G, Chaves A, Mayer J, Rao I . The high level of aluminum resistance in signalgrass is not associated with known mechanisms of external aluminum detoxification in root apices. Plant Physiol. 2001; 125(3):1473-84. PMC: 65625. DOI: 10.1104/pp.125.3.1473. View

14.

BLUMENKRANTZ N, ASBOE-HANSEN G . New method for quantitative determination of uronic acids. Anal Biochem. 1973; 54(2):484-9. DOI: 10.1016/0003-2697(73)90377-1. View

15.

Ma J, Hiradate S, Nomoto K, Iwashita T, Matsumoto H . Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves). Plant Physiol. 1997; 113(4):1033-1039. PMC: 158226. DOI: 10.1104/pp.113.4.1033. View

16.

Klug B, Horst W . Oxalate exudation into the root-tip water free space confers protection from aluminum toxicity and allows aluminum accumulation in the symplast in buckwheat (Fagopyrum esculentum). New Phytol. 2010; 187(2):380-391. DOI: 10.1111/j.1469-8137.2010.03288.x. View

17.

Pellet D, Papernik L, Kochian L . Multiple Aluminum-Resistance Mechanisms in Wheat (Roles of Root Apical Phosphate and Malate Exudation). Plant Physiol. 1996; 112(2):591-597. PMC: 157982. DOI: 10.1104/pp.112.2.591. View

18.

Yang J, Li Y, Zhang Y, Zhang S, Wu Y, Wu P . Cell wall polysaccharides are specifically involved in the exclusion of aluminum from the rice root apex. Plant Physiol. 2007; 146(2):602-11. PMC: 2245838. DOI: 10.1104/pp.107.111989. View

19.

Pineros M, Shaff J, Manslank H, Alves V, Kochian L . Aluminum resistance in maize cannot be solely explained by root organic acid exudation. A comparative physiological study. Plant Physiol. 2004; 137(1):231-41. PMC: 548854. DOI: 10.1104/pp.104.047357. View

20.

Li Y, Yang J, Zhang Y, Zheng S . Disorganized distribution of homogalacturonan epitopes in cell walls as one possible mechanism for aluminium-induced root growth inhibition in maize. Ann Bot. 2009; 104(2):235-41. PMC: 2710910. DOI: 10.1093/aob/mcp123. View