Tissue Specific Characterisation of Lim-kinase 1 Expression During Mouse Embryogenesis

Overview

Journal Gene Expr Patterns

Publisher Elsevier

Date 2010 Dec 21

PMID 21167960

Citations 8

Authors

Nils O Lindstrom

Carlos Neves

Rebecca McIntosh

Zosia Miedzybrodzka

Neil Vargesson

J Martin Collinson

Affiliations

Soon will be listed here.

Abstract

The Lim-kinase (LIMK) proteins are important for the regulation of the actin cytoskeleton, in particular the control of actin nucleation and depolymerisation via regulation of cofilin, and hence may control a large number of processes during development, including cell tensegrity, migration, cell cycling, and axon guidance. LIMK1/LIMK2 knockouts disrupt spinal cord morphogenesis and synapse formation but other tissues and developmental processes that require LIMK are yet to be fully determined. To identify tissues and cell-types that may require LIMK, we characterised the pattern of LIMK1 protein during mouse embryogenesis. We showed that LIMK1 displays an expression pattern that is temporally dynamic and tissue-specific. In several tissues LIMK1 is detected in cell-types that also express Wilms' tumour protein 1 and that undergo transitions between epithelial and mesenchymal states, including the pleura, epicardium, kidney nephrons, and gonads. LIMK1 was also found in a subset of cells in the dorsal retina, and in mesenchymal cells surrounding the peripheral nerves. This detailed study of the spatial and temporal expression of LIMK1 shows that LIMK1 expression is more dynamic than previously reported, in particular at sites of tissue-tissue interactions guiding multiple developmental processes.

Citing Articles

LIM kinases in cardiovascular health and disease.

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Regulation and signaling of the LIM domain kinases.

Casanova-Sepulveda G, Boggon T Bioessays. 2024; 47(1):e2400184.

PMID: 39361252 PMC: 11663136. DOI: 10.1002/bies.202400184.

The Role of LIM Kinases during Development: A Lens to Get a Glimpse of Their Implication in Pathologies.

Ribba A, Fraboulet S, Sadoul K, Lafanechere L Cells. 2022; 11(3).

PMID: 35159213 PMC: 8834001. DOI: 10.3390/cells11030403.

A quantitative proteomic analysis of cofilin phosphorylation in myeloid cells and its modulation using the LIM kinase inhibitor Pyr1.

Prudent R, Demoncheaux N, Diemer H, Collin-Faure V, Kapur R, Paublant F PLoS One. 2018; 13(12):e0208979.

PMID: 30550596 PMC: 6294390. DOI: 10.1371/journal.pone.0208979.

The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse.

Collinson J, Lindstrom N, Neves C, Wallace K, Meharg C, Charles R Development. 2018; 145(3).

PMID: 29439133 PMC: 5818009. DOI: 10.1242/dev.160093.

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