YeeV is an Escherichia Coli Toxin That Inhibits Cell Division by Targeting the Cytoskeleton Proteins, FtsZ and MreB

Overview

Journal Mol Microbiol

Specialties Microbiology
Molecular Biology

Date 2010 Dec 21

PMID 21166897

Citations 76

Authors

Qian Tan

Naoki Awano

Masayori Inouye

Affiliations

Soon will be listed here.

Abstract

Toxin-antitoxin (TA) systems of free-living bacteria have recently demonstrated that these toxins inhibit cell growth by targeting essential functions of cellular metabolism. Here we show that YeeV toxin inhibits cell division, leads to a change in morphology and lysis of Escherichia coli cells. YeeV interacts with two essential cytoskeleton proteins, FtsZ and MreB. Purified YeeV inhibits both the GTPase activity and the GTP-dependent polymerization of FtsZ. YeeV also inhibits ATP-dependent polymerization of MreB. Truncated C-terminal deletions of YeeV result in elongation of cells, and a deletion of the first 15 amino acids from the N-terminus of YeeV caused lemon-shaped cell formation. The YeeV toxin is distinct from other well-studied toxins: it directs the binding of two cytoskeletal proteins and inhibits FtsZ and MreB simultaneously.

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