Pathway Analysis of Liver Metabolism Under Stressed Condition

Overview

Journal J Theor Biol

Publisher Elsevier

Specialty Biology

Date 2010 Dec 18

PMID 21163266

Citations 8

Authors

Mehmet A Orman

Francois Berthiaume

Ioannis P Androulakis

Marianthi G Ierapetritou

Affiliations

Soon will be listed here.

Abstract

Pathway analysis is a useful tool which reveals important metabolic network properties. However, the big challenge is to propose an objective function for estimating active pathways, which represent the actual state of network. In order to provide weight values for all possible pathways within the metabolic network, this study presents different approaches, considering the structural and physiological properties of the metabolic network, aiming at a unique decomposition of the flux vector into pathways. These methods were used to analyze the hepatic metabolism considering available data sets obtained from the perfused livers of fasted rats receiving burn injury. Utilizing unique decomposition techniques and different fluxes revealed that higher weights were always attributed to short pathways. Specific pathways, including pyruvate, glutamate and oxaloacetate pools, and urea production from arginine, were found to be important or essential in all methods and experimental conditions. Moreover the pathways, including serine production from glycine and conversion between acetoacetate and B-OH-butyrate, were assigned higher weights. Pathway analysis was also used to identify the main sources for the production of certain products in the hepatic metabolic network to gain a better understanding of the effects of burn injury on liver metabolism.

Citing Articles

Optimal Temperature Protocols for Liver Machine Perfusion Using a Monte Carlo Method.

Lucia A, Uygun K IFAC Pap OnLine. 2024; 55(23):35-40.

PMID: 38410830 PMC: 10895677. DOI: 10.1016/j.ifacol.2023.01.011.

Modeling energy depletion in rat livers using Nash equilibrium metabolic pathway analysis.

Lucia A, Ferrarese E, Uygun K Sci Rep. 2022; 12(1):3496.

PMID: 35241684 PMC: 8894355. DOI: 10.1038/s41598-022-06966-2.

Hypoxia tolerance, longevity and cancer-resistance in the mole rat Spalax - a liver transcriptomics approach.

Schmidt H, Malik A, Bicker A, Poetzsch G, Avivi A, Shams I Sci Rep. 2017; 7(1):14348.

PMID: 29084988 PMC: 5662568. DOI: 10.1038/s41598-017-13905-z.

Stoichiometry based steady-state hepatic flux analysis: computational and experimental aspects.

Orman M, Mattick J, Androulakis I, Berthiaume F, Ierapetritou M Metabolites. 2014; 2(1):268-91.

PMID: 24957379 PMC: 3901202. DOI: 10.3390/metabo2010268.

Resuscitation of ischemic donor livers with normothermic machine perfusion: a metabolic flux analysis of treatment in rats.

Izamis M, Tolboom H, Uygun B, Berthiaume F, Yarmush M, Uygun K PLoS One. 2013; 8(7):e69758.

PMID: 23922793 PMC: 3724866. DOI: 10.1371/journal.pone.0069758.

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