Replication and Transcription of Human Papillomavirus Type 58 Genome in Saccharomyces Cerevisiae

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2010 Dec 16

PMID 21156081

Citations 1

Authors

Jing Li

Xiao Wang

Juan Liu

Hong Wang

Xiao-Li Zhang

Wei Tang

Yun-dong Sun

Xin Wang

Xiu-Ping Yu

Wei-Ming Zhao

Affiliations

Soon will be listed here.

Abstract

Background: To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast.

Results: HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated.

Conclusions: E2 protein could participate in viral genome maintenance, replication and transcription regulation. This yeast model could be used for the study of certain aspects of HPV life cycle.

Citing Articles

Production of Polyclonal Antibody to the HPV58 E7 Protein and Its Detection in Cervical Cancer.

Zheng Q, Wang T, Jiang S, Han R, Jin N, Zhu J PLoS One. 2016; 11(12):e0169138.

PMID: 28033368 PMC: 5199089. DOI: 10.1371/journal.pone.0169138.

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