Further Characterization of the Impact of Ethanol on βLH: Alterations in Polyribosome Association of βLH MRNA

Overview

Journal Endocrine

Specialty Endocrinology

Date 2010 Dec 15

PMID 21153252

Citations 1

Authors

M M Halloran

M A Emanuele

N V Emanuele

J J Tentler

M R Kelley

Affiliations

Soon will be listed here.

Abstract

We have previously reported a decrease in Luteinizing Hormone (LH) levels in serum afterin vivo acute ethanol exposure in male rats. Accompanying these changes, a rapid and marked decrease of β-LH mRNA was observed. A similar decrease was not detected in the common α-subunit or β-FSH mRNA. The studies presented here examined the possible mechanisms of decreasing β-LH mRNA by using S1 nuclease protection assay to evaluate the effect of acute ethanol exposure on the levels of β-LH heteronuclear RNA (hnRNA). There was no significant difference detected in the level of β-LH hnRNA after ethanol exposure. Polysome distribution analysis was used to evaluate the association and disassociation of β-LH mRNA with polyribosomes since non-polyribosome associated mRNA may be more vulnerable to degradation by RNAases. The results indicated a decrease in the association of the β-LH mRNA with polysomes following acute ethanol exposure. This decrease in polyribosome association would increase the exposure of the β-LH transcript making it more susceptible to RNases. We conclude that the decrease in steady-state β-LH mRNA levels after ethanol exposure occurs because of increasing degradation of the transcript rendered vulnerable by displacement from polysomes and not through a decreased transcriptional rate.

Citing Articles

Impact and reversibility of chronic ethanol feeding on the reproductive axis in the peripubertal male rat.

Emanuele N, LaPaglia N, VOGL W, Steiner J, Kirsteins L, Emanuele M Endocrine. 2000; 11(3):277-84.

PMID: 10786824 DOI: 10.1385/ENDO:11:3:277.

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