The Effect of Two Different Polyethylene Glycol (PEG) Derivatives on the Immunological Response of PEG Grafted Pancreatic Islets

Overview

Journal J Artif Organs

Specialty Biomedical Engineering

Date 2010 Dec 4

PMID 21127930

Citations 5

Authors

Hamideh Aghajani-Lazarjani

Ebrahim Vasheghani-Farahani

Seyed Abbas Shojaosadati

Sameereh Hashemi-Najafabadi

Saleh Zahediasl

Taki Tiraihi

Fatemeh Atyabi

Affiliations

Soon will be listed here.

Abstract

Islet transplantation is one of the promising ways to treat diabetes. To reduce the immune system response, several methods have been developed, a novel one being the grafting of methoxy polyethylene glycol (mPEG) derivatives onto collagen capsules of islets. In this study, the effects of the first and second generations of activated mPEG on the immunological response of polyethylene glycol (PEG) grafted pancreatic islets were studied. mPEG-Succinimidyl carbonate (mPEG-SC) and mPEG-succinimidyl propionic acid (mPEG-SPA) (with nominal molecular weight 5 kDa), typical of the first and second generations of activated mPEG, were selected, respectively. Both activated mPEGs did not affect the morphology, viability, or functionality of PEGylated islets compared to free islets (naked islets). The amount of IL-2 secreted from lymphocytes co-cultured with mPEG-SPA grafted islets (131.83 ± 15.28 pg/ml) was not significantly different from that with mPEG-SC grafted islets (156.09 ± 27.94 pg/ml). These results indicated that both mPEG-SC and mPEG-SPA had the same effect for camouflaging Langerhans islets, but the former is more suitable due to its easier synthesis process.

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