Membrane Trafficking Mediated by OsDRP2B is Specific for Cellulose Biosynthesis

Overview

Journal Plant Signal Behav

Specialty Biology

Date 2010 Dec 4

PMID 21127404

Citations 4

Authors

Rui Li

Guangyan Xiong

Yihua Zhou

Affiliations

Soon will be listed here.

Abstract

Increasing evidence has revealed that membrane trafficking is highly associated with cell wall metabolism. Factors involved in vesicle delivery, e.g. cytoskeleton and motor proteins, have showed regulatory effects on cell wall structure and components. However, little is known about the involvement of other trafficking components in distribution of cell wall-related compartments. Dynamins are important proteins functioning in membrane tubulation and vesiculation. Recently, we have reported characterization of the rice dynamin-related protein 2B (OsDRP2B). Mutation in OsDRP2B causes a significant reduction in cellulose content. Its association with the trans-Golgi network (TGN) and clathrin-coated vesicles and the reduced CESA4 abundance at the bc3 plasma membrane suggest that BC3/OsDRP2B is involved in the transport of essential elements for cellulose synthesis. Here, we provide additional evidence for BC3 subcellular localization via observing OsDRP2B-GFP in living root hairs of transgenic plants. Uronic acid and fractional composition analyses further confirm that the amount of arabinoxylan and other noncellulosic polysaccharides is increased in bc3. However, three putative xylan synthesis genes are down-regulated in mutant plant revealed by real-time PCR analysis. These results imply that compartments delivered by OsDRP2B are specifically responsible for cellulose biosynthesis.

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References

Collings D, Gebbie L, Howles P, Hurley U, Birch R, Cork A . Arabidopsis dynamin-like protein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion. J Exp Bot. 2008; 59(2):361-76. DOI: 10.1093/jxb/erm324. View

Lucas W, Lee J . Plasmodesmata as a supracellular control network in plants. Nat Rev Mol Cell Biol. 2004; 5(9):712-26. DOI: 10.1038/nrm1470. View

Zhang B, Deng L, Qian Q, Xiong G, Zeng D, Li R . A missense mutation in the transmembrane domain of CESA4 affects protein abundance in the plasma membrane and results in abnormal cell wall biosynthesis in rice. Plant Mol Biol. 2009; 71(4-5):509-24. DOI: 10.1007/s11103-009-9536-4. View

Bosca S, Barton C, Taylor N, Ryden P, Neumetzler L, Pauly M . Interactions between MUR10/CesA7-dependent secondary cellulose biosynthesis and primary cell wall structure. Plant Physiol. 2006; 142(4):1353-63. PMC: 1676063. DOI: 10.1104/pp.106.087700. View

Pena M, Zhong R, Zhou G, Richardson E, ONeill M, Darvill A . Arabidopsis irregular xylem8 and irregular xylem9: implications for the complexity of glucuronoxylan biosynthesis. Plant Cell. 2007; 19(2):549-63. PMC: 1867335. DOI: 10.1105/tpc.106.049320. View

Wightman R, Turner S . The roles of the cytoskeleton during cellulose deposition at the secondary cell wall. Plant J. 2008; 54(5):794-805. DOI: 10.1111/j.1365-313X.2008.03444.x. View

Lynch M, Staehelin L . Domain-specific and cell type-specific localization of two types of cell wall matrix polysaccharides in the clover root tip. J Cell Biol. 1992; 118(2):467-79. PMC: 2290055. DOI: 10.1083/jcb.118.2.467. View

Robert S, Bichet A, Grandjean O, Kierzkowski D, Satiat-Jeunemaitre B, Pelletier S . An Arabidopsis endo-1,4-beta-D-glucanase involved in cellulose synthesis undergoes regulated intracellular cycling. Plant Cell. 2005; 17(12):3378-89. PMC: 1315376. DOI: 10.1105/tpc.105.036228. View

Gutierrez R, Lindeboom J, Paredez A, Emons A, Ehrhardt D . Arabidopsis cortical microtubules position cellulose synthase delivery to the plasma membrane and interact with cellulose synthase trafficking compartments. Nat Cell Biol. 2009; 11(7):797-806. DOI: 10.1038/ncb1886. View

10.

Persson S, Caffall K, Freshour G, Hilley M, Bauer S, Poindexter P . The Arabidopsis irregular xylem8 mutant is deficient in glucuronoxylan and homogalacturonan, which are essential for secondary cell wall integrity. Plant Cell. 2007; 19(1):237-55. PMC: 1820957. DOI: 10.1105/tpc.106.047720. View

11.

Ovecka M, Lang I, Baluska F, Ismail A, Illes P, Lichtscheidl I . Endocytosis and vesicle trafficking during tip growth of root hairs. Protoplasma. 2005; 226(1-2):39-54. DOI: 10.1007/s00709-005-0103-9. View

12.

Lukowitz W, Nickle T, Meinke D, Last R, Conklin P, Somerville C . Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis. Proc Natl Acad Sci U S A. 2001; 98(5):2262-7. PMC: 30126. DOI: 10.1073/pnas.051625798. View

13.

Xiong G, Li R, Qian Q, Song X, Liu X, Yu Y . The rice dynamin-related protein DRP2B mediates membrane trafficking, and thereby plays a critical role in secondary cell wall cellulose biosynthesis. Plant J. 2010; 64(1):56-70. DOI: 10.1111/j.1365-313X.2010.04308.x. View

14.

Brown D, Goubet F, Wong V, Goodacre R, Stephens E, Dupree P . Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis. Plant J. 2007; 52(6):1154-68. DOI: 10.1111/j.1365-313X.2007.03307.x. View

15.

Willats W, Orfila C, Limberg G, Buchholt H, Van Alebeek G, Voragen A . Modulation of the degree and pattern of methyl-esterification of pectic homogalacturonan in plant cell walls. Implications for pectin methyl esterase action, matrix properties, and cell adhesion. J Biol Chem. 2001; 276(22):19404-13. DOI: 10.1074/jbc.M011242200. View

16.

Lauber M, Waizenegger I, Steinmann T, Schwarz H, Mayer U, Hwang I . The Arabidopsis KNOLLE protein is a cytokinesis-specific syntaxin. J Cell Biol. 1998; 139(6):1485-93. PMC: 2132613. DOI: 10.1083/jcb.139.6.1485. View

17.

Hepler P, Vidali L, Cheung A . Polarized cell growth in higher plants. Annu Rev Cell Dev Biol. 2001; 17:159-87. DOI: 10.1146/annurev.cellbio.17.1.159. View

18.

Johansen J, Vernhettes S, Hofte H . The ins and outs of plant cell walls. Curr Opin Plant Biol. 2006; 9(6):616-20. DOI: 10.1016/j.pbi.2006.09.007. View

19.

Crowell E, Bischoff V, Desprez T, Rolland A, Stierhof Y, Schumacher K . Pausing of Golgi bodies on microtubules regulates secretion of cellulose synthase complexes in Arabidopsis. Plant Cell. 2009; 21(4):1141-54. PMC: 2685615. DOI: 10.1105/tpc.108.065334. View

20.

Takei K, Yoshida Y, Yamada H . Regulatory mechanisms of dynamin-dependent endocytosis. J Biochem. 2005; 137(3):243-7. DOI: 10.1093/jb/mvi052. View