Foamy Retrovirus Integrase Contains a Pol Dimerization Domain Required for Protease Activation

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2010 Dec 3

PMID 21123385

Citations 12

Authors

Eun-Gyung Lee

Jacqueline Roy

Dana Jackson

Patrick Clark

Paul L Boyer

Stephen H Hughes

Maxine L Linial

Affiliations

Soon will be listed here.

Abstract

Unlike orthoretroviruses, foamy retroviruses (FV) synthesize Pol independently of Gag. The FV Pol precursor is cleaved only once between reverse transcriptase (RT) and integrase (IN) by the protease (PR), resulting in a PR-RT and an IN protein. Only the Pol precursor, not the cleaved subunits, is packaged into virions. Like orthoretroviral PRs, FV PR needs to dimerize to be active. Previously, we showed that a Pol mutant lacking IN has defects in PR activity and Pol packaging into virions. We now show that introduction of a leucine zipper (zip) dimerization motif in an IN truncation mutant can restore PR activity, leading to Pol processing in cells. However, these zip mutants neither cleave Gag nor incorporate Pol into virions. We propose that IN is required for Pol dimerization, which is necessary for the creation of a functional PR active site.

Citing Articles

Cryo-EM structure of the HIV-1 Pol polyprotein provides insights into virion maturation.

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Crystal Structure of a Retroviral Polyprotein: Prototype Foamy Virus Protease-Reverse Transcriptase (PR-RT).

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The Unique, the Known, and the Unknown of Spumaretrovirus Assembly.

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Foamy Virus Integrase in Development of Viral Vector for Gene Therapy.

Kim J, Lee G, Shin C J Microbiol Biotechnol. 2020; 30(9):1273-1281.

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Foamy Virus Protein-Nucleic Acid Interactions during Particle Morphogenesis.

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