Antimicrobial Activity of Bacillus Amyloliquefaciens LBM 5006 is Enhanced in the Presence of Escherichia Coli

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2010 Nov 25

PMID 21104084

Citations 7

Authors

Lisianne Benitez

AnaPaula Correa

Daniel Daroit

Adriano Brandelli

Affiliations

Soon will be listed here.

Abstract

Increased antimicrobial activity was observed when Bacillus amyloliquefaciens LBM 5006 strain was cultivated in the presence of thermally inactivated cells of Escherichia coli, but not with Staphylococcus aureus, Listeria monocytogenes, or Bacillus cereus. E. coli also enhanced the antimicrobial activity when it was added to the medium in the form of living cells or as cell debris after cellular fractionation. No inducing activity was observed with addition of cell-free supernatant of E. coli cultures, suggesting that inducing factor is associated to the cells. Polyacrylamide gel electrophoresis revealed that additional peptide bands are secreted when B. amyloliquefaciens was cultivated in the presence of cell debris of E. coli. These results suggest that the presence of intact or inactivated E. coli enhanced the synthesis of antimicrobial peptides by B. amyloliquefaciens LBM 5006.

Citing Articles

Antimicrobial Activity of BS4 against Gram-Negative Pathogenic Bacteria.

Palacios-Rodriguez A, Espinoza-Culupu A, Duran Y, Sanchez-Rojas T Antibiotics (Basel). 2024; 13(4).

PMID: 38666980 PMC: 11047741. DOI: 10.3390/antibiotics13040304.

Enhancing chemical and biological diversity by co-cultivation.

Selegato D, Castro-Gamboa I Front Microbiol. 2023; 14:1117559.

PMID: 36819067 PMC: 9928954. DOI: 10.3389/fmicb.2023.1117559.

In Vitro and In Vivo Anti- Effect of a Probiotic Strain.

Islam M, Seo H, Redwan A, Kim S, Lee S, Siddiquee M J Microbiol Biotechnol. 2021; 32(1):46-55.

PMID: 34675143 PMC: 9628829. DOI: 10.4014/jmb.2107.07057.

spp. Isolated from Puba as a Source of Biosurfactants and Antimicrobial Lipopeptides.

Perez K, Viana J, Lopes F, Pereira J, Dos Santos D, Oliveira J Front Microbiol. 2017; 8:61.

PMID: 28197131 PMC: 5281586. DOI: 10.3389/fmicb.2017.00061.

Secondary metabolites from Bacillus amyloliquefaciens isolated from soil can kill Burkholderia pseudomallei.

Boottanun P, Potisap C, Hurdle J, Sermswan R AMB Express. 2017; 7(1):16.

PMID: 28050857 PMC: 5209304. DOI: 10.1186/s13568-016-0302-0.

References

Cotter P, Hill C, Ross R . Bacteriocins: developing innate immunity for food. Nat Rev Microbiol. 2005; 3(10):777-88. DOI: 10.1038/nrmicro1273. View

Stein T . Bacillus subtilis antibiotics: structures, syntheses and specific functions. Mol Microbiol. 2005; 56(4):845-57. DOI: 10.1111/j.1365-2958.2005.04587.x. View

Maldonado A, Ruiz-Barba J, Jimenez-Diaz R . Production of plantaricin NC8 by Lactobacillus plantarum NC8 is induced in the presence of different types of gram-positive bacteria. Arch Microbiol. 2003; 181(1):8-16. DOI: 10.1007/s00203-003-0606-8. View

Miller M, Bassler B . Quorum sensing in bacteria. Annu Rev Microbiol. 2001; 55:165-99. DOI: 10.1146/annurev.micro.55.1.165. View

Lisboa M, Bonatto D, Bizani D, Henriques J, Brandelli A . Characterization of a bacteriocin-like substance produced by Bacillus amyloliquefaciens isolated from the Brazilian Atlantic forest. Int Microbiol. 2006; 9(2):111-8. View

Maldonado A, Jimenez-Diaz R, Ruiz-Barba J . Induction of plantaricin production in Lactobacillus plantarum NC8 after coculture with specific gram-positive bacteria is mediated by an autoinduction mechanism. J Bacteriol. 2004; 186(5):1556-64. PMC: 344433. DOI: 10.1128/JB.186.5.1556-1564.2004. View

Nilsen T, Nes I, Holo H . An exported inducer peptide regulates bacteriocin production in Enterococcus faecium CTC492. J Bacteriol. 1998; 180(7):1848-54. PMC: 107099. DOI: 10.1128/JB.180.7.1848-1854.1998. View

Marahiel M, Nakano M, Zuber P . Regulation of peptide antibiotic production in Bacillus. Mol Microbiol. 1993; 7(5):631-6. DOI: 10.1111/j.1365-2958.1993.tb01154.x. View

Mcauliffe O, Ross R, Hill C . Lantibiotics: structure, biosynthesis and mode of action. FEMS Microbiol Rev. 2001; 25(3):285-308. DOI: 10.1111/j.1574-6976.2001.tb00579.x. View

10.

Rojo-Bezares B, Saenz Y, Navarro L, Zarazaga M, Ruiz-Larrea F, Torres C . Coculture-inducible bacteriocin activity of Lactobacillus plantarum strain J23 isolated from grape must. Food Microbiol. 2007; 24(5):482-91. DOI: 10.1016/j.fm.2006.09.003. View

11.

Papagianni M . Ribosomally synthesized peptides with antimicrobial properties: biosynthesis, structure, function, and applications. Biotechnol Adv. 2003; 21(6):465-99. DOI: 10.1016/s0734-9750(03)00077-6. View

12.

Bizani D, Dominguez A, Brandelli A . Purification and partial chemical characterization of the antimicrobial peptide cerein 8A. Lett Appl Microbiol. 2005; 41(3):269-73. DOI: 10.1111/j.1472-765X.2005.01748.x. View

13.

Cherif A, Rezgui W, Raddadi N, Daffonchio D, Boudabous A . Characterization and partial purification of entomocin 110, a newly identified bacteriocin from Bacillus thuringiensis subsp. Entomocidus HD110. Microbiol Res. 2009; 163(6):684-92. DOI: 10.1016/j.micres.2006.10.005. View

14.

Beukes M, Bierbaum G, Sahl H, Hastings J . Purification and partial characterization of a murein hydrolase, millericin B, produced by Streptococcus milleri NMSCC 061. Appl Environ Microbiol. 2000; 66(1):23-8. PMC: 91780. DOI: 10.1128/AEM.66.1.23-28.2000. View

15.

Bowdish D, Davidson D, Hancock R . A re-evaluation of the role of host defence peptides in mammalian immunity. Curr Protein Pept Sci. 2005; 6(1):35-51. DOI: 10.2174/1389203053027494. View

16.

Chang J, Lee H, Chang H . Identification of the agent from Lactobacillus plantarum KFRI464 that enhances bacteriocin production by Leuconostoc citreum GJ7. J Appl Microbiol. 2007; 103(6):2504-15. DOI: 10.1111/j.1365-2672.2007.03543.x. View

17.

Sturme M, Kleerebezem M, Nakayama J, Akkermans A, Vaugha E, de Vos W . Cell to cell communication by autoinducing peptides in gram-positive bacteria. Antonie Van Leeuwenhoek. 2002; 81(1-4):233-43. DOI: 10.1023/a:1020522919555. View

18.

Riley M, Wertz J . Bacteriocins: evolution, ecology, and application. Annu Rev Microbiol. 2002; 56:117-37. DOI: 10.1146/annurev.micro.56.012302.161024. View

19.

Ennahar S, Sashihara T, Sonomoto K, Ishizaki A . Class IIa bacteriocins: biosynthesis, structure and activity. FEMS Microbiol Rev. 2000; 24(1):85-106. DOI: 10.1111/j.1574-6976.2000.tb00534.x. View

20.

Barefoot S, Chen Y, Hughes T, Bodine A, Shearer M, Hughes M . Identification and purification of a protein that induces production of the Lactobacillus acidophilus bacteriocin lactacin B. Appl Environ Microbiol. 1994; 60(10):3522-8. PMC: 201849. DOI: 10.1128/aem.60.10.3522-3528.1994. View