The Putative Lipid Transporter, Arv1, is Required for Activating Pheromone-induced MAP Kinase Signaling in Saccharomyces Cerevisiae

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2010 Nov 25

PMID 21098723

Citations 10

Authors

Michelle L Villasmil

Alison Ansbach

Joseph T Nickels Jr

Affiliations

Soon will be listed here.

Abstract

Saccharomyces cerevisiae haploid cells respond to extrinsic mating signals by forming polarized projections (shmoos), which are necessary for conjugation. We have examined the role of the putative lipid transporter, Arv1, in yeast mating, particularly the conserved Arv1 homology domain (AHD) within Arv1 and its role in this process. Previously it was shown that arv1 cells harbor defects in sphingolipid and glycosylphosphatidylinositol (GPI) biosyntheses and may harbor sterol trafficking defects. Here we demonstrate that arv1 cells are mating defective and cannot form shmoos. They lack the ability to initiate pheromone-induced G1 cell cycle arrest, due to failure to polarize PI(4,5)P(2) and the Ste5 scaffold, which results in weakened MAP kinase signaling activity. A mutant Ste5, Ste5(Q59L), which binds more tightly to the plasma membrane, suppresses the MAP kinase signaling defects of arv1 cells. Filipin staining shows arv1 cells contain altered levels of various sterol microdomains that persist throughout the mating process. Data suggest that the sterol trafficking defects of arv1 affect PI(4,5)P(2) polarization, which causes a mislocalization of Ste5, resulting in defective MAP kinase signaling and the inability to mate. Importantly, our studies show that the AHD of Arv1 is required for mating, pheromone-induced G1 cell cycle arrest, and for sterol trafficking.

Citing Articles

Arv1; a "Mover and Shaker" of Subcellular Lipids.

Corbalan J, Frietze K, Nickels J, Sturley S Contact (Thousand Oaks). 2025; 8:25152564251314601.

PMID: 39845563 PMC: 11748065. DOI: 10.1177/25152564251314601.

An Erg11 lanosterol 14-α-demethylase-Arv1 complex is required for Candida albicans virulence.

Villasmil M, Barbosa A, Cunningham J, Siniossoglou S, Nickels Jr J PLoS One. 2020; 15(7):e0235746.

PMID: 32678853 PMC: 7367482. DOI: 10.1371/journal.pone.0235746.

Mice lacking have reduced signs of metabolic syndrome and non-alcoholic fatty liver disease.

Gallo-Ebert C, Francisco J, Liu H, Draper R, Modi K, Hayward M J Biol Chem. 2018; 293(16):5956-5974.

PMID: 29491146 PMC: 5912475. DOI: 10.1074/jbc.RA117.000800.

A link between very long chain fatty acid elongation and mating-specific yeast cell cycle arrest.

Villasmil M, Gallo-Ebert C, Liu H, Francisco J, Nickels Jr J Cell Cycle. 2017; 16(22):2192-2203.

PMID: 28745545 PMC: 5736334. DOI: 10.1080/15384101.2017.1329065.

Proper Sterol Distribution Is Required for Hyphal Formation and Virulence.

McCourt P, Liu H, Parker J, Gallo-Ebert C, Donigan M, Bata A G3 (Bethesda). 2016; 6(11):3455-3465.

PMID: 27587298 PMC: 5100844. DOI: 10.1534/g3.116.033969.

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