Temporal and Functional Analysis of DNA Replicated in Early S Phase

Overview

Journal Adv Enzyme Regul

Specialty Biochemistry

Date 2010 Nov 25

PMID 21093474

Citations 2

Authors

David G Kaufman

Stephanie M Cohen

Paul D Chastain

Affiliations

Soon will be listed here.

Abstract

In summary, recently developed technologies have begun to draw back the curtain of mystery that obscures some of the basic mechanisms of DNA replication at multiple levels. Studies using extended DNA and chromatin fiber techniques have proven valuable for identifying the location of origins of replication at specific genomic sites and determining their temporal order of replication, for identifying and quantifying sites of DNA damage and localizing chromatin proteins in relation to sites of DNA replication. The future potential of these methods include further discoveries in functional genomics and contributions to the elucidation of the histone code. Such studies could prove very valuable in studies of the mechanisms of cancer development, aging, and other processes of disordered genomic functioning.

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Application of the microfluidic-assisted replication track analysis to measure DNA repair in human and mouse cells.

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