Three-dimensional Scaffolds for Tissue Engineering: the Importance of Uniformity in Pore Size and Structure

Overview

Journal Langmuir

Publisher American Chemical Society

Specialty Chemistry

Date 2010 Nov 25

PMID 21090781

Citations 45

Authors

Sung-Wook Choi

Yu Zhang

Younan Xia

Affiliations

Soon will be listed here.

Abstract

To validate the importance of uniformity in pore size and structure of a scaffold for tissue engineering, we fabricated two types of scaffolds with uniform (inverse opal scaffolds) and nonuniform pore sizes and structures, and then evaluated their properties in terms of diffusion of macromolecules, spatial distribution of fibroblasts, and differentiation of preosteoblasts. Our results confirmed the superior performance of the inverse opal scaffolds due to the uniform pore size, homogeneous environment, and high interconnectivity: a higher diffusion rate, a uniform distribution of cells, and a higher degree of differentiation. In addition, we found that both the differentiation of cells and secretion of extracellular matrix were dependent on the properties of the individual pore to which the cells were attached, rather than the bulk properties of a scaffold. Our results clearly indicate that inverse opal scaffolds could provide a better microenvironment for cells in comparison to a scaffold with nonuniform size and structure.

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References

Flemming R, Murphy C, Abrams G, Goodman S, Nealey P . Effects of synthetic micro- and nano-structured surfaces on cell behavior. Biomaterials. 1999; 20(6):573-88. DOI: 10.1016/s0142-9612(98)00209-9. View

Zhang K, Yan H, Bell D, Stein A, Francis L . Effects of materials parameters on mineralization and degradation of sol-gel bioactive glasses with 3D-ordered macroporous structures. J Biomed Mater Res A. 2003; 66(4):860-9. DOI: 10.1002/jbm.a.10093. View

Kuboki Y, Jin Q, Takita H . Geometry of carriers controlling phenotypic expression in BMP-induced osteogenesis and chondrogenesis. J Bone Joint Surg Am. 2001; 83-A Suppl 1(Pt 2):S105-15. View

Webster T, Ergun C, Doremus R, Siegel R, Bizios R . Enhanced functions of osteoblasts on nanophase ceramics. Biomaterials. 2000; 21(17):1803-10. DOI: 10.1016/s0142-9612(00)00075-2. View

Yuan H, Kurashina K, de Bruijn J, Li Y, de Groot K, Zhang X . A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics. Biomaterials. 1999; 20(19):1799-806. DOI: 10.1016/s0142-9612(99)00075-7. View

Sontjens S, Nettles D, Carnahan M, Setton L, Grinstaff M . Biodendrimer-based hydrogel scaffolds for cartilage tissue repair. Biomacromolecules. 2006; 7(1):310-6. DOI: 10.1021/bm050663e. View

Srouji S, Kizhner T, Livne E . 3D scaffolds for bone marrow stem cell support in bone repair. Regen Med. 2007; 1(4):519-28. DOI: 10.2217/17460751.1.4.519. View

Li S, de Wijn J, Li J, Layrolle P, de Groot K . Macroporous biphasic calcium phosphate scaffold with high permeability/porosity ratio. Tissue Eng. 2003; 9(3):535-48. DOI: 10.1089/107632703322066714. View

Li X, Xie J, Yuan X, Xia Y . Coating electrospun poly(epsilon-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering. Langmuir. 2008; 24(24):14145-50. DOI: 10.1021/la802984a. View

10.

Chang B, Lee C, Hong K, Youn H, Ryu H, Chung S . Osteoconduction at porous hydroxyapatite with various pore configurations. Biomaterials. 2000; 21(12):1291-8. DOI: 10.1016/s0142-9612(00)00030-2. View

11.

Radisic M, Euloth M, Yang L, Langer R, Freed L, Vunjak-Novakovic G . High-density seeding of myocyte cells for cardiac tissue engineering. Biotechnol Bioeng. 2003; 82(4):403-14. DOI: 10.1002/bit.10594. View

12.

Choi S, Cheong I, Kim J, Xia Y . Preparation of uniform microspheres using a simple fluidic device and their crystallization into close-packed lattices. Small. 2009; 5(4):454-9. DOI: 10.1002/smll.200801498. View

13.

Mauney J, Blumberg J, Pirun M, Volloch V, Vunjak-Novakovic G, Kaplan D . Osteogenic differentiation of human bone marrow stromal cells on partially demineralized bone scaffolds in vitro. Tissue Eng. 2004; 10(1-2):81-92. DOI: 10.1089/107632704322791727. View

14.

Botchwey E, Dupree M, Pollack S, Levine E, Laurencin C . Tissue engineered bone: measurement of nutrient transport in three-dimensional matrices. J Biomed Mater Res A. 2003; 67(1):357-67. DOI: 10.1002/jbm.a.10111. View

15.

Grimm M, Williams J . Measurements of permeability in human calcaneal trabecular bone. J Biomech. 1997; 30(7):743-5. DOI: 10.1016/s0021-9290(97)00016-x. View

16.

Wang Y, Wu Q, Chen J, Chen G . Evaluation of three-dimensional scaffolds made of blends of hydroxyapatite and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) for bone reconstruction. Biomaterials. 2004; 26(8):899-904. DOI: 10.1016/j.biomaterials.2004.03.035. View

17.

Godbey W, Hindy S, Sherman M, Atala A . A novel use of centrifugal force for cell seeding into porous scaffolds. Biomaterials. 2004; 25(14):2799-805. DOI: 10.1016/j.biomaterials.2003.09.056. View

18.

Zhang K, Washburn N, Simon Jr C . Cytotoxicity of three-dimensionally ordered macroporous sol-gel bioactive glass (3DOM-BG). Biomaterials. 2005; 26(22):4532-9. DOI: 10.1016/j.biomaterials.2004.11.030. View

19.

Nieponice A, Soletti L, Guan J, Deasy B, Huard J, Wagner W . Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique. Biomaterials. 2007; 29(7):825-33. PMC: 2354918. DOI: 10.1016/j.biomaterials.2007.10.044. View

20.

Choi S, Xie J, Xia Y . Chitosan-Based Inverse Opals: Three-Dimensional Scaffolds with Uniform Pore Structures for Cell Culture. Adv Mater. 2009; 21(29):2997-3001. PMC: 2731433. DOI: 10.1002/adma.200803504. View