Induction of Interleukin-1 from Murine Peritoneal Macrophages by Pseudomonas Aeruginosa Exotoxin A

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1990 Apr 1

PMID 2108093

Citations 14

Authors

M L Misfeldt

P K Legaard

S E Howell

M H Fornella

R D LeGrand

Affiliations

Soon will be listed here.

Abstract

Pseudomonas exotoxin A, an ADP-ribosylating toxin produced by Pseudomonas aeruginosa, has been shown to stimulate the proliferation of murine thymocytes, which requires the participation of accessory cells. This requirement for accessory cells can be replaced by supernatant from adherent peritoneal exudate cells that have been stimulated with exotoxin A. Antibody to exotoxin A inhibits the induction of the thymocyte mitogenic activity from adherent peritoneal macrophages. However, antibody to exotoxin A had no effect on the thymocyte proliferation if the antibody was added to supernatant which contained thymocyte mitogenic activity. The thymocyte mitogenic activity was associated with a protein or protein complex with a molecular mass of greater than 10,000 daltons. D10 bioassays indicated the presence of interleukin-1 (IL-1) in the supernatant. Antibody to IL-1 inhibited the ability of supernatant to induce thymocytes to proliferate. Therefore, these data suggest that Pseudomonas exotoxin A can stimulate the production of IL-1 from adherent peritoneal cells, which induces murine thymocytes to proliferate.

Citing Articles

Stimulation of innate immunity by susceptible and multidrug-resistant Pseudomonas aeruginosa: an in vitro and in vivo study.

Giamarellos-Bourboulis E, Plachouras D, Tzivra A, Kousoulas V, Bolanos N, Raftogiannis M Clin Exp Immunol. 2004; 135(2):240-6.

PMID: 14738451 PMC: 1808952. DOI: 10.1111/j.1365-2249.2003.02365.x.

Generation of neutralizing antipeptide antibodies to the enzymatic domain of Pseudomonas aeruginosa exotoxin A.

Elzaim H, Chopra A, Peterson J, Goodheart R, Heggers J Infect Immun. 1998; 66(5):2170-9.

PMID: 9573104 PMC: 108178. DOI: 10.1128/IAI.66.5.2170-2179.1998.

The involvement of macrophage-derived tumour necrosis factor and lipoxygenase products on the neutrophil recruitment induced by Clostridium difficile toxin B.

Souza M, Melo-Filho A, Rocha M, Lyerly D, Cunha F, Lima A Immunology. 1997; 91(2):281-8.

PMID: 9227329 PMC: 1363859. DOI: 10.1046/j.1365-2567.1997.00243.x.

Porins of Pseudomonas aeruginosa induce release of tumor necrosis factor alpha and interleukin-6 by human leukocytes.

Cusumano V, Tufano M, Mancuso G, Carbone M, Rossano F, Fera M Infect Immun. 1997; 65(5):1683-7.

PMID: 9125547 PMC: 175197. DOI: 10.1128/iai.65.5.1683-1687.1997.

In vivo production of exotoxin A and its role in endogenous Pseudomonas aeruginosa septicemia in mice.

Hirakata Y, Furuya N, Tateda K, Kaku M, Yamaguchi K Infect Immun. 1993; 61(6):2468-73.

PMID: 8500881 PMC: 280870. DOI: 10.1128/iai.61.6.2468-2473.1993.

References

UNANUE E, Allen P . The basis for the immunoregulatory role of macrophages and other accessory cells. Science. 1987; 236(4801):551-7. DOI: 10.1126/science.2437650. View

Kaye J, Gillis S, Mizel S, Shevach E, Malek T, Dinarello C . Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J Immunol. 1984; 133(3):1339-45. View

Holt P, Misfeldt M . Induction of an immune response in athymic nude mice to thymus-dependent antigens by Pseudomonas aeruginosa exotoxin A. Cell Immunol. 1985; 95(2):265-75. DOI: 10.1016/0008-8749(85)90314-4. View

Holt P, Misfeldt M . Variables which affect suppression of the immune response induced by Pseudomonas aeruginosa exotoxin A. Infect Immun. 1986; 52(1):96-100. PMC: 262203. DOI: 10.1128/iai.52.1.96-100.1986. View

MacDonald H, Schneider R, Lees R, Howe R, Acha-Orbea H, Festenstein H . T-cell receptor V beta use predicts reactivity and tolerance to Mlsa-encoded antigens. Nature. 1988; 332(6159):40-5. DOI: 10.1038/332040a0. View

Holt P, Misfeldt M . Biological effects of Pseudomonas aeruginosa exotoxin A: lymphoproliferation of T lymphocytes in athymic mice. Eur J Epidemiol. 1988; 4(1):25-32. DOI: 10.1007/BF00152688. View

Fleischer B, Schrezenmeier H . T cell stimulation by staphylococcal enterotoxins. Clonally variable response and requirement for major histocompatibility complex class II molecules on accessory or target cells. J Exp Med. 1988; 167(5):1697-707. PMC: 2188941. DOI: 10.1084/jem.167.5.1697. View

White J, Herman A, Pullen A, Kubo R, Kappler J, Marrack P . The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell. 1989; 56(1):27-35. DOI: 10.1016/0092-8674(89)90980-x. View

Bauer A, Rutenfranz I, Kirchner H . Processing requirements for T cell activation by Mycoplasma arthritidis-derived mitogen. Eur J Immunol. 1988; 18(12):2109-12. DOI: 10.1002/eji.1830181239. View

10.

Janeway Jr C, Yagi J, Conrad P, Katz M, Jones B, Vroegop S . T-cell responses to Mls and to bacterial proteins that mimic its behavior. Immunol Rev. 1989; 107:61-88. DOI: 10.1111/j.1600-065x.1989.tb00003.x. View

11.

Janeway C, Chalupny J, Conrad P, Buxser S . An external stimulus that mimics Mls locus responses. J Immunogenet. 1988; 15(1-3):161-8. DOI: 10.1111/j.1744-313x.1988.tb00418.x. View

12.

Helle M, Boeije L, Aarden L . IL-6 is an intermediate in IL-1-induced thymocyte proliferation. J Immunol. 1989; 142(12):4335-8. View

13.

Scholl P, Diez A, Mourad W, Parsonnet J, Geha R, Chatila T . Toxic shock syndrome toxin 1 binds to major histocompatibility complex class II molecules. Proc Natl Acad Sci U S A. 1989; 86(11):4210-4. PMC: 287420. DOI: 10.1073/pnas.86.11.4210. View

14.

Kappler J, Kotzin B, Herron L, Gelfand E, Bigler R, Boylston A . V beta-specific stimulation of human T cells by staphylococcal toxins. Science. 1989; 244(4906):811-3. DOI: 10.1126/science.2524876. View

15.

Mollick J, Cook R, Rich R . Class II MHC molecules are specific receptors for staphylococcus enterotoxin A. Science. 1989; 244(4906):817-20. DOI: 10.1126/science.2658055. View

16.

Bauer A, Giese M, Kirchner H . Role of interleukin 1 in mycoplasma mitogen-induced proliferation of human T cells. Immunobiology. 1989; 179(1):124-30. DOI: 10.1016/S0171-2985(89)80011-7. View

17.

UNANUE E . The regulatory role of macrophages in antigenic stimulation. Part Two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol. 1981; 31:1-136. DOI: 10.1016/s0065-2776(08)60919-0. View

18.

Holt P, Misfeldt M . Alteration of murine immune response by Pseudomonas aeruginosa exotoxin A. Infect Immun. 1984; 45(1):227-33. PMC: 263305. DOI: 10.1128/iai.45.1.227-233.1984. View

19.

Calvano S, Quimby F, Antonacci A, Reiser R, Bergdoll M, Dineen P . Analysis of the mitogenic effects of toxic shock toxin on human peripheral blood mononuclear cells in vitro. Clin Immunol Immunopathol. 1984; 33(1):99-110. DOI: 10.1016/0090-1229(84)90296-4. View