Immunohistochemical Study of the Abnormal Cells in Langerhans Cell Histiocytosis (histiocytosis X)

Overview

Journal Virchows Arch A Pathol Anat Histopathol

Specialty Anatomy & Histology

Date 1990 Jan 1

PMID 2107627

Citations 6

Authors

K Ornvold

E Ralfkiaer

H Carstensen

Affiliations

Soon will be listed here.

Abstract

The immunophenotypic properties of the abnormal cells in routine specimens from 16 cases of Langerhans cell histiocytosis (LCH) were examined. In five cases, cryostat sections were also available. The abnormal cells expressed a similar phenotype and were positive for HLA-DR, S-100 protein, peanut agglutinin (PNA), CD1a, CD4 and several macrophage-associated markers, including CD11c, CDw32 and CD68 (the latter detectable in routine sections with antibody KP1). Staining with CD14, CD35 (C3b receptor), and CD11b (C3bi receptor) was negative with the exception of one of the cases in which a proportion of the cells showed faint positivity with CD11b. Staining for pan-T-cell (CD2, CD3, CD5) and pan-B-cell (CD19, CD22) antigens was negative in all lesions. It is concluded that LCH expresses a characteristic phenotype with some heterogeneity with regard to macrophage markers and that immunohistochemical methods in cryostat sections and routine specimens form a useful supplement to other techniques for the diagnosis of this condition.

Citing Articles

Langerhans Cell Histiocytosis Shows Distinct Cytoplasmic Expression of Major Histocompatibility Class II Antigens.

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Neither IL-17A mRNA nor IL-17A protein are detectable in Langerhans cell histiocytosis lesions.

Peters T, McClain K, Allen C Mol Ther. 2011; 19(8):1433-9.

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Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells.

Allen C, Li L, Peters T, Leung H, Yu A, Man T J Immunol. 2010; 184(8):4557-67.

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Differential Expression of Markers in Extensive and Restricted Langerhans Cell Histiocytosis (LCH).

Kohalmi F, Strausz J, Egervary M, Szekeres G, Timar J Pathol Oncol Res. 1996; 2(3):184-187.

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Expression of the monoclonal antibody HECA-452 defined E-selectin ligands in Langerhans cell histiocytosis.

Simonitsch I, Kopp C, Mosberger I, Volc-Platzer B, Radaszkiewicz T Virchows Arch. 1996; 427(5):477-81.

PMID: 8624576 DOI: 10.1007/BF00199507.

References

Hall P, ODoherty C, Levison D . Langerhans cell histiocytosis: an unusual case illustrating the value of immunohistochemistry in diagnosis. Histopathology. 1987; 11(11):1181-91. DOI: 10.1111/j.1365-2559.1987.tb01858.x. View

Ralfkiaer E, Pulford K, Lauritzen A, Avnstrom S, Guldhammer B, Mason D . Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples. Histopathology. 1989; 14(6):637-43. DOI: 10.1111/j.1365-2559.1989.tb02205.x. View

Ralfkiaer E, Saati T, Bosq J, Delsol G, Gatter K, Mason D . Immunocytochemical characterisation of cutaneous lymphomas other than mycosis fungoides. J Clin Pathol. 1986; 39(5):553-63. PMC: 499919. DOI: 10.1136/jcp.39.5.553. View

Norton A, Isaacson P . Lymphoma phenotyping in formalin-fixed and paraffin wax-embedded tissues. I. Range of antibodies and staining patterns. Histopathology. 1989; 14(5):437-46. DOI: 10.1111/j.1365-2559.1989.tb02180.x. View

Murphy G, Harrist T, Bhan A, Mihm Jr M . Distribution of cell surface antigens in histiocytosis X cells. Quantitative immunoelectron microscopy using monoclonal antibodies. Lab Invest. 1983; 48(1):90-7. View

LAHEY E . Histiocytosis x--an analysis of prognostic factors. J Pediatr. 1975; 87(2):184-9. DOI: 10.1016/s0022-3476(75)80576-2. View

Warnke R, Pulford K, Pallesen G, Ralfkiaer E, Brown D, Gatter K . Diagnosis of myelomonocytic and macrophage neoplasms in routinely processed tissue biopsies with monoclonal antibody KP1. Am J Pathol. 1989; 135(6):1089-95. PMC: 1880478. View

Barbey S, Gane P, Le Pelletier O, NEZELOF C . Histiocytosis X Langerhans cells react with antiinterleukin-2 receptor monoclonal antibody. Pediatr Pathol. 1987; 7(5-6):569-74. DOI: 10.3109/15513818709161420. View

Meyer A, Steinmeier T, Loning T, Radzun H, Delling G . Histiocytic differentiation in benign and malignant bone tumors. J Cancer Res Clin Oncol. 1988; 114(6):565-74. DOI: 10.1007/BF00398178. View

10.

Ree H, Kadin M . Peanut agglutinin. A useful marker for histiocytosis X and interdigitating reticulum cells. Cancer. 1986; 57(2):282-7. DOI: 10.1002/1097-0142(19860115)57:2<282::aid-cncr2820570216>3.0.co;2-q. View

11.

Davey F, Gatter K, Ralfkiaer E, Pulford K, Krissansen G, Mason D . Immunophenotyping of non-Hodgkin's lymphomas using a panel of antibodies on paraffin-embedded tissues. Am J Pathol. 1987; 129(1):54-63. PMC: 1899695. View

12.

Groh V, Gadner H, Radaszkiewicz T, Rappersberger K, Konrad K, Wolff K . The phenotypic spectrum of histiocytosis X cells. J Invest Dermatol. 1988; 90(4):441-7. DOI: 10.1111/1523-1747.ep12460878. View

13.

Bos J, Sillevis Smitt J, Krieg S, BAKKER P, Vos G, VAN ZAANE D . Acute disseminated histiocytosis-X: in situ immunophenotyping with monoclonal antibodies. J Cutan Pathol. 1984; 11(1):59-64. DOI: 10.1111/j.1600-0560.1984.tb00350.x. View

14.

Ralfkiaer E, STEIN H, Ralfkiaer N, HOU-JENSEN K, Mason D . Normal and neoplastic Langerhans cells: phenotypic comparison with other types of macrophages. Adv Exp Med Biol. 1985; 186:1009-15. DOI: 10.1007/978-1-4613-2463-8_122. View

15.

Kelly P, Bliss E, MORTON J, Burns J, Mcgee J . Monoclonal antibody EBM/11: high cellular specificity for human macrophages. J Clin Pathol. 1988; 41(5):510-5. PMC: 1141502. DOI: 10.1136/jcp.41.5.510. View

16.

Uchiyama T, Broder S, Waldmann T . A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells. J Immunol. 1981; 126(4):1393-7. View

17.

Ruco L, Remotti D, Monardo F, Uccini S, Cristiani M, Modesti A . Letterer-Siwe disease: immunohistochemical evidence for a proliferative disorder involving immature cells of Langerhans lineage. Virchows Arch A Pathol Anat Histopathol. 1988; 413(3):239-47. DOI: 10.1007/BF00718616. View

18.

Cordell J, Falini B, Erber W, Ghosh A, Abdulaziz Z, MacDonald S . Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984; 32(2):219-29. DOI: 10.1177/32.2.6198355. View

19.

. Histiocytosis syndromes in children. Writing Group of the Histiocyte Society. Lancet. 1987; 1(8526):208-9. View

20.

Davey F, Cordell J, Erber W, Pulford K, Gatter K, Mason D . Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol. 1988; 41(7):753-8. PMC: 1141583. DOI: 10.1136/jcp.41.7.753. View