The Drosophila Jing Gene is a Downstream Target in the Trachealess/Tango Tracheal Pathway

Overview

Journal Dev Genes Evol

Specialty Biology

Date 2010 Nov 10

PMID 21061019

Citations 1

Authors

Tatiana Morozova

Joanne Hackett

Yalda Sedaghat

Margaret Sonnenfeld

Affiliations

Soon will be listed here.

Abstract

Primary branching in the Drosophila trachea is regulated by the Trachealess (Trh) and Tango (Tgo) basic helix-loop-helix-PAS (bHLH-PAS) heterodimers, the POU protein Drifter (Dfr)/Ventral Veinless (Vvl), and the Pointed (Pnt) ETS transcription factor. The jing gene encodes a zinc finger protein also required for tracheal development. Three Trh/Tgo DNA-binding sites, known as CNS midline elements, in 1.5 kb of jing 5′ cis-regulatory sequence (jing1.5) previously suggested a downstream role for jing in the pathway. Here, we show that jing is a direct downstream target of Trh/Tgo and that Vvl and Pnt are also involved in jing tracheal activation. In vivo lacZ enhancer detection assays were used to identify cis-regulatory elements mediating embryonic expression patterns of jing. A 2.8-kb jing enhancer (jing2.8) drove lacZ expression in all tracheal cell lineages, the CNS midline and Engrailed-positive segmental stripes, mimicking endogenous jing expression. A 1.3-kb element within jing2.8 drove expression that was restricted to Engrailed-positive CNS midline cells and segmental ectodermal stripes. Surprisingly, jing1.5-lacZ expression was restricted to tracheal fusion cells despite the presence of consensus DNA-binding sites for bHLH-PAS, ETS, and POU domain transcription factors. Given the absence of Trh/Tgo DNA-binding sites in the jing1.3 enhancer, these results are consistent with previous observations suggesting a combinatorial basis to Trh-/Tgo-mediated transcriptional regulation in the trachea.

Citing Articles

Crossveinless is a direct transcriptional target of Trachealess and Tango in Drosophila tracheal precursor cells.

Lovato C, Lovato T, M Cripps R PLoS One. 2019; 14(6):e0217906.

PMID: 31158257 PMC: 6546244. DOI: 10.1371/journal.pone.0217906.

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