Stimulatory Effect of Echinacea Purpurea Extract on the Trafficking Activity of Mouse Dendritic Cells: Revealed by Genomic and Proteomic Analyses

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2010 Nov 3

PMID 21040561

Citations 12

Authors

Shu-Yi Yin

Wen-Hsin Wang

Bi-Xue Wang

Kandan Aravindaram

Pei-Ing Hwang

Han-Ming Wu

Ning-Sun Yang

Affiliations

Soon will be listed here.

Abstract

Background: Several Echinacea species have been used as nutraceuticals or botanical drugs for "immunostimulation", but scientific evidence supporting their therapeutic use is still controversial. In this study, a phytocompound mixture extracted from the butanol fraction (BF) of a stem and leaf (S+L) extract of E. purpurea ([BF/S+L/Ep]) containing stringently defined bioactive phytocompounds was obtained using standardized and published procedures. The transcriptomic and proteomic effects of this phytoextract on mouse bone marrow-derived dendritic cells (BMDCs) were analyzed using primary cultures.

Results: Treatment of BMDCs with [BF/S+L/Ep] did not significantly influence the phenotypic maturation activity of dendritic cells (DCs). Affymetrix DNA microarray and bioinformatics analyses of genes differentially expressed in DCs treated with [BF/S+L/Ep] for 4 or 12 h revealed that the majority of responsive genes were related to cell adhesion or motility (Cdh10, Itga6, Cdh1, Gja1 and Mmp8), or were chemokines (Cxcl2, Cxcl7) or signaling molecules (Nrxn1, Pkce and Acss1). TRANSPATH database analyses of gene expression and related signaling pathways in treated-DCs predicted the JNK, PP2C-α, AKT, ERK1/2 or MAPKAPK pathways as the putative targets of [BF/S+L/Ep]. In parallel, proteomic analysis showed that the expressions of metabolic-, cytoskeleton- or NF-κB signaling-related proteins were regulated by treatment with [BF/S+L/Ep]. In vitro flow cytometry analysis of chemotaxis-related receptors and in vivo cell trafficking assay further showed that DCs treated with [BF/S+L/Ep] were able to migrate more effectively to peripheral lymph node and spleen tissues than DCs treated as control groups.

Conclusion: Results from this study suggest that [BF/S+L/Ep] modulates DC mobility and related cellular physiology in the mouse immune system. Moreover, the signaling networks and molecules highlighted here are potential targets for nutritional or clinical application of Echinacea or other candidate medicinal plants.

Citing Articles

On the Bioactivity of Extracts to Modulate the Production of Inflammatory Mediators.

Vieira S, M F Goncalves V, Llaguno C, Macias F, Tiritan M, Reis R Int J Mol Sci. 2022; 23(21).

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Cellular and Molecular Signaling as Targets for Cancer Vaccine Therapeutics.

Wei W, Shyur L, Yang N Cells. 2022; 11(9).

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Echinacea purpurea (L.) Moench treatment of monocytes promotes tonic interferon signaling, increased innate immunity gene expression and DNA repeat hypermethylated silencing of endogenous retroviral sequences.

Declerck K, Perez Novo C, Grielens L, Van Camp G, Suter A, Vanden Berghe W BMC Complement Med Ther. 2021; 21(1):141.

PMID: 33980308 PMC: 8114977. DOI: 10.1186/s12906-021-03310-5.

Developing Phytocompounds from Medicinal Plants as Immunomodulators.

Wen C, Chen H, Yang N Adv Bot Res. 2020; 62:197-272.

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Application of proteomics in the elucidation of chemical-mediated allergic contact dermatitis.

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