Diagnostic Performance of Gastric Imprint Smear for Determination of Helicobacter Pylori Infection

Overview

Journal Can J Gastroenterol

Specialty Gastroenterology

Date 2010 Nov 2

PMID 21037989

Citations 1

Authors

Jaber Al-Ali

Fahad Al-Asfar

Rita Dhar

Piyaray M Dhar

Kapila Kusum

Affiliations

Soon will be listed here.

Abstract

Background: Despite the availability of several methods (invasive and noninvasive) for the diagnosis of Helicobacter pylori infection, no test is considered to be the 'gold standard'. Endoscopy-based tests are regarded as the reference method in most studies.

Objective: To evaluate the diagnostic performance of imprint cytology smears of antral biopsies compared with Gram-stained smears, the rapid urease test and culture methods, separately and in combination.

Methods: Antral biopsies were obtained from consecutive patients undergoing upper gastrointestinal endoscopy at a single centre. The biopsies were examined for the presence of H pylori by Gram-stained smear, the rapid urease test, culture methods and imprint cytology smear. 

Results: A total of 273 biopsies were studied. All tests were positive in 36% of the patients. Of 252 biopsies tested, 73% were positive using the imprint cytology technique. Using Gram-stained smear, the rapid urease test and culture methods individually, the sensitivity and specificity of imprint cytology smears for the detection of H pylori were found to be 92.7% and 50%; 92.7% and 49%; and 92.4% and 38.5%, respectively. Combining the three microbiological methods resulted in a sensitivity of 92.1%, a specificity of 51.0% and an efficiency of 71.7% for imprint cytology smears.

Conclusions: Endoscopic examination provides useful clinical information. Imprint gastric cytology can be used as a rapid test to establish the diagnosis of H pylori infection at the time endoscopy is performed, enabling the endoscopist to start treatment with immediate effect.

Citing Articles

Evaluation of methods for H. pylori detection in PPI consumption using culture, rapid urease test and smear examination.

Siavoshi F, Saniee P, Khalili-Samani S, Hosseini F, Malakutikhah F, Mamivand M Ann Transl Med. 2015; 3(1):11.

PMID: 25705643 PMC: 4293475. DOI: 10.3978/j.issn.2305-5839.2014.11.16.

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