Microbial Stimulation Fully Differentiates Monocytes to DC-SIGN/CD209(+) Dendritic Cells for Immune T Cell Areas

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2010 Oct 30

PMID 21029863

Citations 326

Authors

Cheolho Cheong

Ines Matos

Jae-Hoon Choi

Durga Bhavani Dandamudi

Elina Shrestha

M Paula Longhi

Kate L Jeffrey

Robert M Anthony

Courtney Kluger

Godwin Nchinda

Hyein Koh

Anthony Rodriguez

Juliana Idoyaga

Maggi Pack

Klara Velinzon

Chae Gyu Park

Ralph M Steinman

Affiliations

Soon will be listed here.

Abstract

Dendritic cells (DCs), critical antigen-presenting cells for immune control, normally derive from bone marrow precursors distinct from monocytes. It is not yet established if the large reservoir of monocytes can develop into cells with critical features of DCs in vivo. We now show that fully differentiated monocyte-derived DCs (Mo-DCs) develop in mice and DC-SIGN/CD209a marks the cells. Mo-DCs are recruited from blood monocytes into lymph nodes by lipopolysaccharide and live or dead gram-negative bacteria. Mobilization requires TLR4 and its CD14 coreceptor and Trif. When tested for antigen-presenting function, Mo-DCs are as active as classical DCs, including cross-presentation of proteins and live gram-negative bacteria on MHC I in vivo. Fully differentiated Mo-DCs acquire DC morphology and localize to T cell areas via L-selectin and CCR7. Thus the blood monocyte reservoir becomes the dominant presenting cell in response to select microbes, yielding DC-SIGN(+) cells with critical functions of DCs.

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