Expression of a Rice Glutelin Promoter in Transgenic Tobacco

Overview

Journal Plant Mol Biol

Date 1990 Jan 1

PMID 2101310

Citations 20

Authors

D J Leisy

J Hnilo

Y Zhao

T W Okita

Affiliations

Soon will be listed here.

Abstract

A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers.

Citing Articles

Tapetum-specific expression of theOsg6B promoter-β-glucuronidase gene in transgenic rice.

Yokoi S, Tsuchiya T, Toriyama K, Hinata K Plant Cell Rep. 2019; 16(6):363-367.

PMID: 30727642 DOI: 10.1007/BF01146774.

A vacuolar antiporter is differentially regulated in leaves and roots of the halophytic wild rice Porteresia coarctata (Roxb.) Tateoka.

Kizhakkedath P, Jegadeeson V, Venkataraman G, Parida A Mol Biol Rep. 2014; 42(6):1091-105.

PMID: 25481774 DOI: 10.1007/s11033-014-3848-4.

Molecular and genetic characterization of elite transgenic rice plants produced by electric-discharge particle acceleration.

Cooley J, Ford T, Christou P Theor Appl Genet. 2013; 90(1):97-104.

PMID: 24173789 DOI: 10.1007/BF00221001.

Overexpression of the oil palm (Elaeis guineensis Jacq.) TAPETUM DEVELOPMENT1-like Eg707 in rice affects cell division and differentiation and reduces fertility.

Thuc L, Geelen D, Ky H, Ooi S, Napis S, Sinniah U Mol Biol Rep. 2012; 40(2):1579-90.

PMID: 23086301 DOI: 10.1007/s11033-012-2206-7.

Promoter diversity in multigene transformation.

Peremarti A, Twyman R, Gomez-Galera S, Naqvi S, Farre G, Sabalza M Plant Mol Biol. 2010; 73(4-5):363-78.

PMID: 20354894 DOI: 10.1007/s11103-010-9628-1.

References

Higuchi W, Fukazawa C . A rice glutelin and a soybean glycinin have evolved from a common ancestral gene. Gene. 1987; 55(2-3):245-53. DOI: 10.1016/0378-1119(87)90284-8. View

Benfey P, Chua N . Regulated genes in transgenic plants. Science. 1989; 244(4901):174-81. DOI: 10.1126/science.244.4901.174. View

Colot V, Robert L, Kavanagh T, Bevan M, Thompson R . Localization of sequences in wheat endosperm protein genes which confer tissue-specific expression in tobacco. EMBO J. 1987; 6(12):3559-64. PMC: 553821. DOI: 10.1002/j.1460-2075.1987.tb02685.x. View

Schernthaner J, Matzke M, Matzke A . Endosperm-specific activity of a zein gene promoter in transgenic tobacco plants. EMBO J. 1988; 7(5):1249-55. PMC: 458370. DOI: 10.1002/j.1460-2075.1988.tb02938.x. View

Voelker T, Sturm A, Chrispeels M . Differences in expression between two seed lectin alleles obtained from normal and lectin-deficient beans are maintained in transgenic tobacco. EMBO J. 1987; 6(12):3571-7. PMC: 553823. DOI: 10.1002/j.1460-2075.1987.tb02687.x. View

Casadaban M, Cohen S . Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980; 138(2):179-207. DOI: 10.1016/0022-2836(80)90283-1. View

Bray E, Naito S, Pan N, Anderson E, Dube P, Beachy R . Expression of the β-subunit of β-conglycinin in seeds of transgenic plants. Planta. 2013; 172(3):364-70. DOI: 10.1007/BF00398665. View

Marris C, Gallois P, Copley J, Kreis M . The 5' flanking region of a barley B hordein gene controls tissue and developmental specific CAT expression in tobacco plants. Plant Mol Biol. 2013; 10(4):359-66. DOI: 10.1007/BF00029886. View

Weising K, Schell J, Kahl G . Foreign genes in plants: transfer, structure, expression, and applications. Annu Rev Genet. 1988; 22:421-77. DOI: 10.1146/annurev.ge.22.120188.002225. View

10.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

11.

Chen Z, Schuler M, Beachy R . Functional analysis of regulatory elements in a plant embryo-specific gene. Proc Natl Acad Sci U S A. 1986; 83(22):8560-4. PMC: 386970. DOI: 10.1073/pnas.83.22.8560. View

12.

Gamborg O, Miller R, Ojima K . Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968; 50(1):151-8. DOI: 10.1016/0014-4827(68)90403-5. View

13.

Barker S, Harada J, Goldberg R . Cellular localization of soybean storage protein mRNA in transformed tobacco seeds. Proc Natl Acad Sci U S A. 1988; 85(2):458-62. PMC: 279569. DOI: 10.1073/pnas.85.2.458. View

14.

Okita T, Hwang Y, Hnilo J, Kim W, Aryan A, Larson R . Structure and expression of the rice glutelin multigene family. J Biol Chem. 1989; 264(21):12573-81. View

15.

Amasino R . Acceleration of nucleic acid hybridization rate by polyethylene glycol. Anal Biochem. 1986; 152(2):304-7. DOI: 10.1016/0003-2697(86)90413-6. View

16.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

17.

Chen Z, Pan N, Beachy R . A DNA sequence element that confers seed-specific enhancement to a constitutive promoter. EMBO J. 1988; 7(2):297-302. PMC: 454308. DOI: 10.1002/j.1460-2075.1988.tb02812.x. View

18.

Ueng P, Galili G, Sapanara V, Goldsbrough P, Dube P, Beachy R . Expression of a maize storage protein gene in petunia plants is not restricted to seeds. Plant Physiol. 1988; 86(4):1281-5. PMC: 1054665. DOI: 10.1104/pp.86.4.1281. View

19.

Sengupta-Gopalan C, Reichert N, Barker R, Hall T, Kemp J . Developmentally regulated expression of the bean beta-phaseolin gene in tobacco seed. Proc Natl Acad Sci U S A. 1985; 82(10):3320-4. PMC: 397767. DOI: 10.1073/pnas.82.10.3320. View